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for global research and industry clients, covering processes from callus induction and regeneration system optimization to Agrobacterium-mediated transformation, transgenic verification, and stable expression. Our services apply to sweet cherry, sour cherry, and various commercial rootstocks, meeting diverse needs including gene function research, disease and stress resistance screening, and quality improvement.
Cherry (Prunus spp.) is an important fruit tree of the Rosaceae family, genus Prunus, widely distributed in Europe, South Asia, and the Himalayan region, with large-scale commercial cultivation established in North America and Australia. Sweet cherry (Prunus avium L.) and sour cherry (Prunus cerasus L.) are the two most representative varieties, with sweet cherry particularly favored by consumers for its excellent taste, rich nutrition, and attractive color. According to FAO data, global annual production exceeds 2 million tons, with Turkey, the United States, and Iran being the main producing countries. Cherry fruits are rich in dietary fiber, potassium, magnesium, vitamin C, carotenoids, anthocyanins, and polyphenols, offering health benefits including antioxidant, anti-inflammatory, and blood pressure-lowering effects. As consumer demands for fruit quality increase, how to enhance cherry disease resistance, storage stability, and nutritional quality through genetic approaches has become a focus of molecular breeding and biotechnology development.
Based on years of plant genetic engineering research experience, Creative Biogene has established an efficient genetic transformation platform suitable for different cherry varieties, covering the following services:
| Service | Description |
| Compatible with multiple cultivated cherry types (sour cherry, sweet cherry, and rootstocks) | Tailored protocols for various cherry genotypes, including CAB-6p sour cherry, sweet cherry cultivars, and commercial rootstocks (e.g., Gisela 6/7, Colt), ensuring broad applicability. |
| Genetic transformation system optimization and development | Optimized explant selection, hormone combinations, selection agents, and co-cultivation conditions significantly enhance transformation and regeneration efficiency. |
| Target gene overexpression and silencing validation | Utilizes strong promoters (e.g., CaMV 35S, Ubiquitin) for overexpression and RNAi or amiRNA strategies for gene silencing to investigate gene functions. |
| Implementation of gene editing strategies such as CRISPR/Cas | Employs CRISPR/Cas9 for precise gene knockout or modification; includes custom gRNA design, off-target analysis, and stable line screening. |
| Superior trait introduction and stable genetic expression | Introduces genes for improved firmness, color, disease resistance, and shelf life, with stable inheritance confirmed across generations. |
| Molecular detection, phenotypic verification, and transgenic plant screening | Offers PCR, qRT-PCR, GUS/GFP, Western/Southern blotting, and phenotype evaluation to identify elite transgenic lines. |
Through modern molecular tools, we can assist clients in achieving the following trait enhancement goals:
| Trait Direction | Improvement Strategy |
| Fruit firmness and shelf life | Regulate pectin metabolism gene expression. |
| Increased anthocyanin content | Upregulate key enzymes such as CHS, DFR, UFGT. |
| Crack resistance | Enhance cell wall structure-related gene expression. |
| Disease resistance | Introduce virus resistance or antimicrobial peptide genes. |
| Nutrient enrichment | Edit metabolic regulatory genes (e.g., Car, Anth, VitC pathways) |
As a highly commercialized fruit crop with extreme quality requirements, genetic improvement of cherry is crucial for enhancing industrial competitiveness. Creative Biogene relies on advanced transformation platforms and rich practical experience to provide efficient and precise technical support for research and industry clients, helping you achieve breakthrough progress in new variety breeding and functional gene analysis. If you have further customization needs, please contact us for detailed service solutions.
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