Muskmelon Transformation Service
Creative Biogene has developed a refined Agrobacterium-mediated transformation system tailored for melon. This platform enables gene overexpression, gene silencing, and genome editing. We offer an end-to-end service pipeline covering vector construction, explant preconditioning, transformation, regeneration, molecular validation, and transgenic line propagation, ensuring reproducibility and success across various melon genotypes.
Melon (Cucumis melo L.), a globally important horticultural crop, is prized for its diverse varieties, unique flavor, and high market value. However, genetic improvement in melon remains challenging due to its low transformation efficiency and high genotype dependence. Creative Biogene provides a comprehensive melon genetic transformation service platform based on optimized Agrobacterium-mediated methods, specifically designed to overcome these limitations. Our service supports efficient gene functional studies, genome editing applications, and trait-specific improvement in melon. Melon belongs to the Cucurbitaceae family and exhibits significant economic and nutritional importance worldwide. Despite the growing interest in gene function studies and molecular breeding, stable genetic transformation in melon is still technically demanding due to issues such as low regeneration efficiency, variety-dependent response, and poor reproducibility.
Service Content
Creative Biogene's melon genetic transformation and genome editing services are built on two robust technical pathways—stable genetic transformation and genome editing—each tailored to support functional genomics, trait improvement, and molecular breeding efforts.
1. Stable Genetic Transformation (Agrobacterium-mediated)
We utilize an optimized Agrobacterium-mediated protocol specifically refined for melon, which includes:
- Cultivar Selection & Explant Optimization
- Use of highly responsive genotypes (e.g., Bawudun, Xinmi hybrids) and cotyledonary nodes as explants, preconditioned with precise hormone combinations to enhance regeneration efficiency.
- Agrobacterium Infection Protocol
- Fine-tuned infection conditions using effective Agrobacterium tumefaciens strains, with controlled OD600 values, vacuum-assisted infiltration, and dark co-culture to maximize T-DNA delivery.
- Selection & Shoot Regeneration
- Rigorous antibiotic screening (e.g., kanamycin, hygromycin) and elimination of Agrobacterium (e.g., cefotaxime treatment), followed by shoot induction on cytokinin-enriched medium.
- Rooting & Acclimatization
- Regenerated shoots are rooted in auxin-supplemented medium and transferred to soil under greenhouse conditions for phenotypic observation and transgenic line propagation.
2. Genome Editing
We offer complete genome editing solutions for targeted manipulation of melon genes, including:
- Custom gRNA Design & Vector Construction
- Bioinformatics-assisted guide RNA design, off-target site analysis, and multi-gRNA cassette assembly for knockout, knock-in, or promoter editing applications.
- Transformation & Editing Execution
- Delivery of editing vectors via our established Agrobacterium-mediated system to obtain edited events in T0 generation plants.
- Editing Verification
- Genotyping of edited loci using PCR and Sanger sequencing, followed by editing efficiency evaluation and optional next-generation sequencing (NGS) for off-target analysis.
- Functional Validation & Line Development
- Expression analysis (qPCR), phenotypic characterization, and homozygous line screening are available upon request
Technical Highlights
- Genotype-Adaptive Protocols: Customized transformation workflow tailored to the specific requirements of different melon cultivars.
- Advanced Screening Strategy: Dual antibiotic screening combined with visual marker tracking to ensure high transformation accuracy.
- CRISPR/Cas9 Integration: Full support for genome editing projects including gene knockout, base editing, and targeted trait improvement.
- Comprehensive Validation: Multiple molecular assays ensure stable transgene integration and expression.
- Rapid Turnaround: Optimized timelines to accelerate research progress from gene to transgenic plant.
Applications
- Functional genomics and gene discovery
- Quality trait improvement (e.g., aroma, sweetness, shelf-life)
- Biotic and abiotic stress tolerance studies
- Metabolic pathway engineering and secondary metabolite production
- Genome editing for precision breeding and cultivar development
Service Workflow
- Project Consultation: Requirement analysis, gene/target discussion, and protocol design
- Vector Construction: Cloning, sgRNA design, and construct assembly
- Transformation: Explant preparation, Agrobacterium infection, co-culture
- Regeneration: Shoot induction, elongation, rooting, and acclimatization
- Validation: PCR, sequencing, qPCR, and phenotypic evaluation
- Delivery: Transgenic plants, technical report, and ongoing support
Case Study
Contact Us
If you are interested in our melon genetic transformation services or need customized support for your research goals, please contact Creative Biogene. Our experienced team will provide full technical assistance from experimental design to data analysis, helping you achieve breakthrough results in melon molecular breeding and gene function studies.
References:
- Kaewsuksaeng S, Wonglom P, Sunpapao A. Electrostatic Atomized Water Particles Induce Disease Resistance in Muskmelon (Cucumis melo L.) against Postharvest Fruit Rot Caused by Fusarium incarnatum. J Fungi (Basel). 2023 Jul 13;9(7):745.
- Wang Y, Zou Z, Su X, et al. Physiological of biochar and α-Fe2O3 nanoparticles as amendments of Cd accumulation and toxicity toward muskmelon grown in pots. J Nanobiotechnology. 2021 Dec 20;19(1):442.
* For research use only. Not intended for any clinical use.
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