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Medicago sativa/truncatula Transformation Service

Based on the comprehensive plant transformation and tissue culture platform established through years of experiences, scientists from Creative Biogene offer Medicago sativa and Medicago truncatula transformation service. Creative Biogene strives to produce a sufficient number of transgenic plants quickly and at minimum cost for academic and commercial.

Medicago truncatula and Medicago sativa have become model systems for legumes due to its relatively small stature, small genome (450–500 Mb), short generation time (about 3 months), and ability to reproduce both by outcrossing and selfing. Besides, Medicago sativa (Alfalfa) has been widely grown as a forage crop due to its good quality characteristics and high adaptability. Glyphosate-resistant alfalfa has become the first deregulated and commercialized transgenic forage crop, which has shown clear advantages in year yield and forage quality comparing with traditional alfalfa. Thus, a rapid transformation system of M. truncatula and M. sativa will facilitate the basic function research of legume plants like soybean, pea and cowpea, as well as increase the yield of forage crop.

Agrobacterium-mediated Medicago truncatula transformation using cotyledon explants. Figure 1. Agrobacterium-mediated Medicago truncatula transformation using cotyledon explants.

Workflow of Medicago truncatula and Medicago sativa transformation

  • Seed sterilization and germination
  • Agrobacterium infection and co-cultivation
  • Selection and plant regeneration
  • Greenhouse care and seed harvest


  • Variety: R108, Jemalong A17.
  • Agrobacterium strain: EHA101, EHA105, GV3101, AGL1, C58C1
  • Selectable marker: hygromycin B, kanamycin, phosphinothricin.

The goal of the Creative Biogene is to provide customers convenient and cost-effective transformation services for pillar species used for basic and applied research. Team members have established efficient transformation protocols for representatives of the Poaceae, Fabaceae and Solanaceae. We offer a complete transformation service from plasmid construction, Agrobacterium-mediated transformation, molecular characterization to functional analysis. For further information, please feel free to contact us at .


  1. Wright, E. (2015). Medicago truncatula transformation using cotyledonary explants. Agrobacterium Protocols.
  2. Wen, L. (2019). Comparison of efficiency and time to regeneration of agrobacterium-mediated transformation methods in medicago truncatula. Plant Methods, 15(1).
For research use only. Not intended for any clinical use.

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