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The dual-reporter stable cell lines provided by Creative Biogene encompass combinations of GFP with luciferase or RFP with luciferase, spanning various cell types. These cell lines serve multiple functions in biological research, including cell signaling pathway analysis, gene expression regulation studies, drug screening, and biological signal detection. Through the dual-reporter system of GFP with luciferase or RFP with luciferase, researchers can monitor real-time cellular dynamics, drug effects, and changes in pathway activity. These cell lines can be utilized to explore intracellular molecular events, assess potential drug candidates, and investigate disease mechanisms, providing powerful tools for scientific research and drug development. Leveraging Creative Biogene's dual-reporter stable cell lines enables comprehensive and in-depth biological studies, thereby accelerating advancements in drug discovery and disease therapeutics.
Advantages of Our Dual Reporter Cell Lines
Dual Reporter Cell Lines are specialized cell models engineered to contain two distinct reporter genes, allowing simultaneous monitoring of multiple cellular processes or molecular events. These cell lines are designed to facilitate various experimental assays by providing a versatile platform for analyzing gene expression, pathway activation, or cellular responses. Dual Reporter Cell Lines can be categorized based on the types of reporter genes integrated into the cell model. Common combinations include luciferase and green fluorescent protein (GFP), luciferase and red fluorescent protein (RFP), or other combinations that enable the detection of different cellular activities or molecular interactions. Common applications of Dual Reporter Cell Lines include:
Case Study 1
As a pivotal information conduit within cells, RNA serves as a potent platform for both interpreting and directing cellular activities. Researchers developed genetically encoded cellular RNA exporters, inspired by viruses, to achieve programmable export of RNA molecules from living cells. This system allows non-destructive monitoring of cell dynamics and facilitates the engineering of cells capable of delivering executable RNA programs to other cells. By efficiently packaging and secreting target RNA molecules within protective nanoparticles, this approach enables the monitoring of cell population dynamics with clonal resolution and facilitates the delivery, expression, and functional activity of exported mRNA in recipient cells. Termed COURIER (Controlled Output and Uptake of RNA for Interrogation, Expression, and Regulation), this technology lays the groundwork for hybrid cell and gene therapies based on cell-to-cell RNA delivery.
Figure 1. The efficacy of delivering Cre-encoding mRNA by EPN24-MCP or Gag-MCP was investigated using the Cre reporter system. RNA was collected from sender cells and their conditioned media were transferred to receiver cells, inducing RFP expression upon Cre recombination. HEK293T cells were transfected with RNA exporter, Cre cargo, and VSV-G fusogen plasmids. The Cre reporter cells, sourced from Creative Biogene, are of the HEK293 Color-Switch loxP/GFP/RFP lineage. (Horns F, et al., 2023)
Case Study 2
Though seemingly simple, finding a safe and effective therapeutic dose and regimen is vital for advancing targeted therapies clinically. Researchers utilize a kinetic-selectivity imaging probe to visualize and quantify the epichaperome, a pathological protein-protein interaction network, aiming to develop diagnostic tools for malignancies. By assessing epichaperome networks and inhibitor engagement in real-time at single-lesion resolution, they optimize dose and schedule selection. This theranostic platform offers preclinical and clinical evidence for precision-medicine targeting of aberrant protein networks, bridging the gap between protein-protein interactions and diagnostic utility.
Figure 2. This process involves establishing tumors in nude mice by injecting MDA-MB-468 breast cancer cells into the forelimbs and NCI-H1975 lung cancer cells into the flank region. Xenografts were generated using NCI-H1975 lung cancer cells expressing luciferase. The Luc/GFP Reporter Cell Line-NCI-H1975 was obtained from Creative Biogene. (Pillarsetty N, et al., 2019)
Q: Can a dual-reporter monitor protein translation levels and occurrence of misfolding?
A: Yes, a dual-reporter system can monitor protein translation levels and occurrence of misfolding. By using two different reporter genes, each reflecting a specific aspect of protein expression and folding, researchers can simultaneously track both translation efficiency and misfolding events within cells. For instance, one reporter gene may indicate the rate of protein synthesis, while another may report on protein misfolding by emitting a fluorescent signal upon misfolded protein accumulation. This enables comprehensive monitoring of protein expression dynamics and quality control mechanisms within biological systems.
Q: Can a dual-reporter system be used as a screening assay?
A: Absolutely, a dual-reporter system is well-suited for use as a screening assay. By employing two distinct reporter genes, each responsive to different cellular events or conditions, researchers can efficiently screen compounds, genetic factors, or environmental conditions for their effects on protein translation and misfolding. This approach allows for rapid identification of potential modulators of these processes, making it highly valuable for screening applications.
Q: How can a dual-reporter system enable high throughput analysis?
A: A dual-reporter system enables high throughput analysis by allowing researchers to monitor multiple cellular events or conditions simultaneously within a single experimental setup. This capability reduces the time and resources required for screening large compound libraries or conducting comprehensive biological studies. Additionally, automated imaging and detection systems can be employed to rapidly analyze the signals generated by the dual-reporter genes in multiple samples, further enhancing throughput.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0060 | GFP/Luciferase Reporter Stable Cell Line - SK-OV-3 | Inquiry |
| CSC-RR00619 | GFP/Luc Reporter Cell Line - Capan-1 | Inquiry |
| CSC-RR00620 | GFP/Luc Reporter Cell Line - Farage | Inquiry |
| CSC-RR00623 | GFP/Luc Reporter Cell Line - WEHI-3 | Inquiry |
| CSC-RR0068 | GFP/RFP Reporter Stable Cell Line-HEK293 | Inquiry |
| CSC-RR0075 | RFP/Luciferase Reporter Stable Cell Line - HEK293 | Inquiry |
| CSC-RR0082 | loxP/GFP/RFP (Puromycin) Color-Switch, CRE Reporter Stable Cell Line-HEK293 | Inquiry |
| CSC-RR00973 | GFP/Luc Reporter Cell Line - 22RV1 | Inquiry |
| CSC-RR00974 | GFP/Luc Reporter Cell Line - U-87MG | Inquiry |
| CSC-RR00975 | GFP/Luc Reporter Cell Line - SIHA | Inquiry |
| CSC-RR00976 | GFP/Luc Reporter Cell Line - RM-1 | Inquiry |
| CSC-RR00977 | GFP/Luc Reporter Cell Line - MB49 | Inquiry |
| CSC-RR00978 | GFP/Luc Reporter Cell Line - LLC | Inquiry |
| CSC-RR00979 | GFP/Luc Reporter Cell Line - Ishikawa | Inquiry |
| CSC-RR00980 | GFP/Luc Reporter Cell Line - PLC/PRF/5 | Inquiry |
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