Luc/RFP Reporter Cell Line-4T1

4T1 is a breast cancer cell line derived from mouse BALB/c strain mammary tissue. 4T1 cells are epithelial cells that are resistant to 6-thioguanine. In preclinical studies, 4T1 cells have been used to study breast cancer metastasis because they can metastasize to the lungs, liver, lymph nodes, brain and bone. 4T1 is similar to human metastatic triple-negative breast cancer (TNBC) and lacks expression of the estrogen receptor (ER), progesterone receptor (PR), and epidermal growth factor receptor 2 (HER2). Furthermore, this cell line is poorly immunogenic in mice, consistent with the characteristics of human breast cancer, resulting in higher tumorigenicity and invasiveness in this breast cancer model. Luc/RFP Reporter Cell Line-4T1 is engineered to stably overexpress Luc (luciferase) and RFP (red fluorescent protein). Luciferase is an enzyme that produces bioluminescence, allowing cells in living animals to be easily detected through bioluminescence imaging. Red fluorescent protein, on the other hand, helps observe the location and spread of cancer cells through fluorescence microscopy. Under the influence of certain substances or conditions, the intensity of fluorescence and bioluminescence produced by these labeled cells increases or decreases. This allows researchers to monitor the effects of various treatments or stimuli on cancer cells in real time.