Luc/GFP Reporter Cell Line-4T1

Name: Luc/GFP Reporter Cell Line-4T1
SKU: CSC-RR0225
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0225

Host Cell : 4T1 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Documents

Cat. No.	CSC-RR0225
Description	Luc/GFP Reporter Cell Line-4T1 is engineered to stably overexpress GFP and luciferase reporter genes. It is an ideal positive control for use in bioluminescence and fluorescence assays. In addition, 4T1 cells form tumors post implantation into immunocompromised mice. The in vivo growth of these tumors can be monitored using bioluminescent imaging or optical imaging for GFP fluorescence.
Target Gene	Luciferase/GFP
Host Cell	4T1
Host Cell Species	Mus musculus (Mouse)
Reporter Type	Fluorescent protein
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

CSC-RR0225-Luc/GFP Reporter Cell Line-4T1-Datasheet

Quick Inquiry

Background

Case Study

Applications

Q & A

Customer Reviews

The Luc/GFP reporter cell line combines the use of luciferase for measuring gene expression and cellular activity with the visualization capabilities of GFP. This dual-reporter system is particularly useful for studying the effects of various treatments or genetic manipulations on both cell viability and gene expression.

The 4T1 cell line, as previously mentioned, is a mouse mammary carcinoma cell line. The Luc/GFP reporter stable cell line in 4T1 provides a versatile tool for cancer research, allowing for the simultaneous monitoring of cell viability and the effects of treatments on gene expression. This cell line is particularly useful for high-throughput screening and for studying the mechanisms of action of potential cancer therapies.

The heterogeneity and genetic adaptation of tumors complicate cancer treatment, especially when relying on targeting specific mutations. Nevertheless, cancer-related pathways such as protein phosphorylation and calcium signaling offer conserved therapeutic targets. Researchers in a recent study demonstrated the potential of a cyclic octapeptide, labeled with a near-infrared dye, to selectively bind to phosphorylated Annexin A2 (pANXA2) in tumor environments with elevated calcium levels. This peptide targets the invasive edges of solid tumors and migrates toward the necrotic core, making it useful for both tumor imaging and drug delivery.

Figure 1 illustrates IHC analyses comparing the distribution of ANXA2/pANXA2 and calcium in different tumor sections, as well as the localization of LS301, a near-infrared fluorescent dye, across various tumor types following its in vivo administration. (doi: 10.1038/s41551-020-0528-7) Figure 1. The researchers used IHC to analyze ANXA2 and pANXA2 distribution in 4T1 tumors. They tracked LS301 peptide distribution using near-infrared fluorescence to explore tumor targeting and GFP fluorescence to monitor tumor cells. 4T1 luc-GFP breast carcinoma cells were used to assess LS301's efficacy in localizing at tumor edges, highlighting its potential for drug delivery and imaging applications. (Shen D, et al., 2020)

In this research, 4T1-Luc/GFP cells were co-cultured with fibroblasts to explore intercellular signaling and molecular transfer mechanisms. Creative Biogene's Luc/GFP Reporter Cell Line - 4T1 could be utilized in similar experimental setups, supporting studies in tumor biology and drug discovery where cell signaling, protein expression, and imaging are critical components.

The Luc/GFP Reporter Cell Line-4T1 offers a powerful platform for studying cancer progression and therapeutic interventions. Derived from mouse mammary gland carcinoma, 4T1 cells exhibit aggressive metastatic behavior, making them an ideal model for investigating metastasis mechanisms and evaluating anti-cancer strategies. The incorporation of both luciferase and green fluorescent protein (GFP) reporters enhances the versatility of this cell line for various applications.

(1)Monitoring Metastatic Spread In Vivo: Luc/GFP Reporter Cell Line-4T1 enables non-invasive tracking of tumor growth and metastasis in live animal models using bioluminescence imaging (BLI) and fluorescence imaging techniques. Researchers can assess the efficacy of therapeutic interventions on inhibiting tumor growth and metastatic dissemination in real-time.

(2)Evaluation of Anti-Metastatic Agents: Researchers utilize Luc/GFP Reporter Cell Line-4T1 to screen and evaluate potential anti-metastatic agents, including small molecules, antibodies, and immunotherapies. The dual reporter system allows for simultaneous assessment of primary tumor growth (luciferase) and metastatic colonization (GFP), facilitating comprehensive preclinical drug testing.

(3)Studying Tumor Microenvironment Interactions: Luc/GFP Reporter Cell Line-4T1 serves as a valuable tool for investigating the interactions between cancer cells and the tumor microenvironment (TME). By visualizing GFP-labeled cancer cells within the context of luciferase-expressing tumors, researchers can elucidate the role of stromal components, immune cells, and extracellular matrix in tumor progression and metastasis.

Customer Q&As

How does the dual-reporter system (Luc/GFP) in the Luc/GFP Reporter Cell Line-4T1 enhance the study of metastatic breast cancer progression in vivo?

A: The dual-reporter system allows for the versatile tracking of 4T1 cells: GFP for real-time fluorescence imaging of cell migration and localization within tissues, and luciferase for sensitive, non-invasive quantification of tumor burden and metastasis via bioluminescence imaging. This combination facilitates comprehensive studies on the dynamics of metastatic spread and tumor growth.

What advantages does the Luc/GFP Reporter Cell Line-4T1 offer for evaluating the efficacy of anti-cancer therapies in preclinical mouse models?

A: This cell line enables the assessment of therapeutic effects on both primary tumor growth and metastatic dissemination. The GFP signal allows for the visualization of microscopic metastases, while the luciferase activity provides quantitative data on overall tumor burden, enabling precise evaluation of treatment efficacy.

Can the Luc/GFP Reporter Cell Line-4T1 be utilized to study tumor microenvironment interactions in metastatic sites?

A: Yes, the GFP fluorescence permits the isolation and detailed examination of 4T1 cells from metastatic sites, facilitating studies on how the tumor microenvironment influences cancer cell behavior. Coupled with the luciferase reporter, this allows for the correlation of microenvironmental factors with tumor growth and metastasis.

How might researchers employ the Luc/GFP Reporter Cell Line-4T1 to investigate the role of specific genes in breast cancer metastasis?

A: Gene function can be explored by genetically modifying the Luc/GFP 4T1 cells to knockdown or overexpress target genes, followed by in vivo tracking of their effects on metastasis using GFP fluorescence and luciferase bioluminescence. This approach helps in identifying genes critical for metastatic progression and potential therapeutic targets.

In what ways could the Luc/GFP Reporter Cell Line-4T1 facilitate the development and testing of novel imaging agents for detecting breast cancer metastases?

A: The cell line's dual reporters make it an ideal model for testing imaging agents' sensitivity and specificity. GFP provides a visual marker for confirming the presence of metastatic cells, while the luciferase signal can be used to test the efficacy of bioluminescence-based imaging agents, enabling the evaluation of new diagnostic tools for early detection of metastases.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Customer Reviews

Dual Reporting Capability

The Luc/GFP Reporter Cell Line-4T1 comes with the ability to report using both luciferase and GFP, providing versatile options for detecting cellular events. This duality allows for flexibility in experimental design, accommodating various detection methods and instruments.

Germany

2020-07-12

Enhanced Visual Tracking

GFP allows for the direct observation of the Luc/GFP Reporter Cell Line-4T1 under a fluorescence microscope, which simplifies the process of cell tracking and localization in complex biological systems.

Canada

2020-10-10

Bioluminescence Quantification

With luciferase, the Luc/GFP Reporter Cell Line-4T1 enables precise quantification of cellular processes, offering a luminometric measure that is more sensitive and less invasive than many other assay types.

Canada

2021-07-16

Non-invasive Readouts

Both luciferase and GFP provide non-invasive readouts, meaning that the Luc/GFP Reporter Cell Line-4T1 can be used in assays where cell viability must be maintained, such as in longitudinal studies or drug efficacy tests.

United States

2023-05-21

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *

Name

Country *

Email *

Related Products

Cat.No.	Product Name	Price
CSC-RR0144	RFP Stable Cell Line-SN12C	Inquiry
CSC-RR0207	Luc/GFP Reporter Cell Line-NCI-H1299	Inquiry
CSC-RR0234	Luc Reporter Cell Line-B16F10	Inquiry
CSC-RR0137	RFP Stable Cell Line-BxPC-3	Inquiry
CSC-RR0216	GFP Reporter Cell Line-NCI-H661	Inquiry
CSC-RR0235	mSlc5a5/GFP Reporter Cell Line-B16F10	Inquiry
CSC-RR0129	GFP Stable Cell Line-NUGC4	Inquiry
CSC-RR0230	mSlc5a5/Luc Reporter Cell Line-4T1	Inquiry
CSC-RR0017	Human TLR3/IFNB Prom/LUCPorter Reporter Stable Cell Line-HEK293	Inquiry
CSC-RR0041	luciferase Reporter Stable Cell Line-HEK293	Inquiry

Luc/GFP Reporter Cell Line-4T1

Cell Line Information

Cell Culture Information

Safety and Packaging

Documents

Background

Case Study

Applications

Q & A

Customer Reviews

How does the dual-reporter system (Luc/GFP) in the Luc/GFP Reporter Cell Line-4T1 enhance the study of metastatic breast cancer progression in vivo?

What advantages does the Luc/GFP Reporter Cell Line-4T1 offer for evaluating the efficacy of anti-cancer therapies in preclinical mouse models?

Can the Luc/GFP Reporter Cell Line-4T1 be utilized to study tumor microenvironment interactions in metastatic sites?

How might researchers employ the Luc/GFP Reporter Cell Line-4T1 to investigate the role of specific genes in breast cancer metastasis?

In what ways could the Luc/GFP Reporter Cell Line-4T1 facilitate the development and testing of novel imaging agents for detecting breast cancer metastases?

Dual Reporting Capability

Enhanced Visual Tracking

Bioluminescence Quantification

Non-invasive Readouts

Related Products

Related Services