Cat.No. : CSC-RR0225 Host Cell: 4T1
| Cat. No. | CSC-RR0225 |
| Description | Luc/GFP Reporter Cell Line-4T1 is engineered to stably overexpress GFP and luciferase reporter genes. It is an ideal positive control for use in bioluminescence and fluorescence assays. In addition, 4T1 cells form tumors post implantation into immunocompromised mice. The in vivo growth of these tumors can be monitored using bioluminescent imaging or optical imaging for GFP fluorescence. |
| Gene | Luciferase/GFP |
| Host Cell | 4T1 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The heterogeneity and genetic adaptation of tumors complicate cancer treatment, especially when relying on targeting specific mutations. Nevertheless, cancer-related pathways such as protein phosphorylation and calcium signaling offer conserved therapeutic targets. Researchers in a recent study demonstrated the potential of a cyclic octapeptide, labeled with a near-infrared dye, to selectively bind to phosphorylated Annexin A2 (pANXA2) in tumor environments with elevated calcium levels. This peptide targets the invasive edges of solid tumors and migrates toward the necrotic core, making it useful for both tumor imaging and drug delivery.
Figure 1. The researchers used IHC to analyze ANXA2 and pANXA2 distribution in 4T1 tumors. They tracked LS301 peptide distribution using near-infrared fluorescence to explore tumor targeting and GFP fluorescence to monitor tumor cells. 4T1 luc-GFP breast carcinoma cells were used to assess LS301's efficacy in localizing at tumor edges, highlighting its potential for drug delivery and imaging applications. (Shen D, et al., 2020)
In this research, 4T1-Luc/GFP cells were co-cultured with fibroblasts to explore intercellular signaling and molecular transfer mechanisms. Creative Biogene's Luc/GFP Reporter Cell Line - 4T1 could be utilized in similar experimental setups, supporting studies in tumor biology and drug discovery where cell signaling, protein expression, and imaging are critical components.
A: The dual-reporter system allows for the versatile tracking of 4T1 cells: GFP for real-time fluorescence imaging of cell migration and localization within tissues, and luciferase for sensitive, non-invasive quantification of tumor burden and metastasis via bioluminescence imaging. This combination facilitates comprehensive studies on the dynamics of metastatic spread and tumor growth.
A: This cell line enables the assessment of therapeutic effects on both primary tumor growth and metastatic dissemination. The GFP signal allows for the visualization of microscopic metastases, while the luciferase activity provides quantitative data on overall tumor burden, enabling precise evaluation of treatment efficacy.
A: Yes, the GFP fluorescence permits the isolation and detailed examination of 4T1 cells from metastatic sites, facilitating studies on how the tumor microenvironment influences cancer cell behavior. Coupled with the luciferase reporter, this allows for the correlation of microenvironmental factors with tumor growth and metastasis.
A: Gene function can be explored by genetically modifying the Luc/GFP 4T1 cells to knockdown or overexpress target genes, followed by in vivo tracking of their effects on metastasis using GFP fluorescence and luciferase bioluminescence. This approach helps in identifying genes critical for metastatic progression and potential therapeutic targets.
A: The cell line's dual reporters make it an ideal model for testing imaging agents' sensitivity and specificity. GFP provides a visual marker for confirming the presence of metastatic cells, while the luciferase signal can be used to test the efficacy of bioluminescence-based imaging agents, enabling the evaluation of new diagnostic tools for early detection of metastases.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Luc/GFP Reporter Cell Line-4T1 comes with the ability to report using both luciferase and GFP, providing versatile options for detecting cellular events. This duality allows for flexibility in experimental design, accommodating various detection methods and instruments.
GFP allows for the direct observation of the Luc/GFP Reporter Cell Line-4T1 under a fluorescence microscope, which simplifies the process of cell tracking and localization in complex biological systems.
With luciferase, the Luc/GFP Reporter Cell Line-4T1 enables precise quantification of cellular processes, offering a luminometric measure that is more sensitive and less invasive than many other assay types.
Both luciferase and GFP provide non-invasive readouts, meaning that the Luc/GFP Reporter Cell Line-4T1 can be used in assays where cell viability must be maintained, such as in longitudinal studies or drug efficacy tests.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
