Cat.No. : CSC-RR0231 Host Cell: B16F10
| Cat. No. | CSC-RR0231 |
| Description | Luc/GFP Reporter Cell Line-B16F10 is engineered to stably co-express GFP and luciferase reporter genes It is an ideal positive control for use in bioluminescence and fluorescence assays. In addition, B16F10 cells can form metastases in mouse lungs. The in vivo growth of metastases can be monitored using bioluminescent imaging or optical imaging for GFP fluorescence. |
| Background | The Luc/GFP reporter cell line-B16F10 has emerged as a pivotal tool in biomedical research |
| Gene | Luciferase/GFP |
| Host Cell | B16F10 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Macrophages play a pivotal role in regulating inflammation and can either promote or inhibit tumor growth and metastasis. Researchers harnessed the anti-tumor potential of macrophages using Ferumoxytol (FMT) combined with the Toll-like receptor 3 (TLR3) agonist, poly (I:C) (PIC), or FP-NPs (nanoparticles composed of FMT-NH2 surface functionalized with PIC). In vitro studies revealed that these combinations synergistically inhibited B16F10 cell proliferation by inducing a tumoricidal macrophage phenotype with increased TNF-α and iNOS expression, enhanced NO secretion, and augmented phagocytosis. In vivo experiments demonstrated regression of primary melanoma and alleviation of pulmonary metastasis with elevated pro-inflammatory macrophage infiltration and upregulation of pro-inflammatory genes. FP-NPs showed enhanced anti-metastatic efficacy by steering macrophages to M1 phenotype through NF-κB signaling.
Figure 1. The combined effects of FMT and PIC on macrophage activation and tumor growth inhibition were studied using Luc/GFP Reporter Cell Line-B16F10. Cell viability, GFP fluorescence intensity, proliferation, and gene expression were analyzed in co-culture experiments with RAW 264.7 cells. The results demonstrated the potential of the FMT/PIC combination in enhancing macrophage activation and inhibiting tumor growth, contributing to the understanding of immune responses in cancer therapy. (Zhao J, et al., 2018)
A: B16F10 cells were chosen for establishing the luciferase/GFP reporter cell line due to their relevance to melanoma research and their well-characterized genetic background. Additionally, B16F10 cells exhibit high transfection efficiency, making them suitable for stable expression studies.
A: The stability and expression level of luciferase and GFP in the B16F10 reporter cell line were confirmed and sustained through stable transfection techniques, antibiotic selection, and validation of expression using luciferase assays and fluorescence microscopy. Regular subculture and monitoring of luciferase activity and fluorescence intensity were employed to maintain stable expression levels.
A: Functional characterization of luciferase and GFP expression in the B16F10 reporter cell line involved assessing their responsiveness to regulatory elements by analyzing changes in luciferase activity and fluorescence intensity in response to stimuli. Additionally, their involvement in cellular processes such as gene regulation, signaling pathways, or drug response was investigated using appropriate assays and imaging techniques.
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Dual fluorescence power! The Luc/GFP Reporter Cell Line in B16F10 cells illuminates research with both luciferase and GFP, offering a dynamic platform for tracking tumor growth and gene expression.
From studying metastasis to evaluating treatment responses, this cell line serves as an invaluable tool for understanding tumor biology and testing novel therapies. The Luc/GFP Reporter Cell Line consistently delivers bright signals, enabling precise monitoring and quantification of cellular events.
Streamlined experimentation! Its dual-reporter system simplifies data collection and interpretation, accelerating discoveries in cancer biology and therapeutics.
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