Luc/GFP Reporter Cell Line-NCI-H1299

Luc/GFP reporter cell line-NCI-H1299 is a stable cell line genetically engineered to express luciferase (Luc) and green fluorescent protein (GFP) under the control of constitutive promoters. These cells have a wide range of applications, particularly in research areas such as cancer, genetics and drug discovery.

Cancer research: This cell line was originally derived from human lung cancer cells, making it an important tool for studying the mechanisms of lung cancer. Researchers can use luciferase and GFP signals to track the growth and metastasis of these cells in vivo and in vitro, helping to improve our understanding of tumor progression.

Drug discovery applications: Luciferase and GFP expression in this cell line can be used as an indicator of the efficacy of drug candidates. Researchers can assess a drug's inhibitory effect on cancer cell proliferation by monitoring the intensity of the luciferase and GFP signals.

Study gene regulation: The luciferase gene can be used as a reporter gene to study the regulation of specific genes. By linking the luciferase gene to a gene of interest, one can monitor the activity of a specific gene based on the light output produced by the luciferase enzyme.

Live cell imaging: GFP expression in this cell line allows cell visualization, allowing researchers to observe cell behaviors such as cell migration, morphological changes, etc.

Toxicity testing: When these cells are exposed to toxic compounds, reductions in luciferase and GFP signals reflect the extent of cell damage.