Cat.No. : CSC-RR0211 Host Cell: NCI-N87
| Cat. No. | CSC-RR0211 |
| Description | Luc/GFP Reporter Cell Line-NCI-N87 is a polyclonal population which is constructed by lentivirus transduction, followed by stable cell selection. This cell line stably expresses luciferase gene under the control of CMV promoter. It also stably expresses G |
| Introduction | Luc/GFP Reporter Cell Line-NCI-N87 is a polyclonal population which is constructed by lentivirus transduction, followed by stable cell selection. This cell line stably expresses luciferase gene under the control of CMV promoter. It also stably expresses GFP and puromycin resistance gene driven by SV40 promoter. This cell line is designed for in vitro or in vivo cancer research. |
| Gene | Luciferase+GFP |
| Host Cell | NCI-N87 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Source | NCI-N87 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
HER2 is a key driver of tumorigenesis in gastric cancer, and trastuzumab is the primary therapy for HER2-positive patients. However, many patients exhibit resistance to trastuzumab due to insufficient drug sensitivity. In the study, the researchers utilized the NCI-N87/luc cell line to establish tumor xenografts in mice, with treatments including trastuzumab and TFBG. This model allowed for the assessment of SHCBP1's role in drug resistance and the evaluation of combination therapy. At Creative Biogene, our Luc/GFP Reporter Cell Line-NCI-N87 can be used for similar research directions, offering a powerful tool for studying gene expression and cell signaling in gastric cancer and other HER2-related research.
Figure 1. The researchers investigated the effects of SHCBP1 knockdown on trastuzumab sensitivity in gastric cancer cell lines NCI-N87 and SNU-216. They performed cell viability assays, colony formation assays, and in vivo xenograft experiments to assess tumor growth and drug sensitivity. Their results demonstrated that SHCBP1 depletion significantly increased trastuzumab sensitivity both in vitro and in vivo. (Shi W, et al., 2021)
A: Our Luc/GFP Reporter Cell Line-NCI-N87 has undergone rigorous screening and verification to ensure stable expression of Luciferase and GFP genes. We verified the expression levels of both through fluorescence microscopy, flow cytometry, and luciferase activity detection, and conducted repeated tests at different time points and in different batches. Cells can maintain stable expression of Luciferase and GFP under appropriate culture conditions.
A: The impact of the NCI-N87 cell line as a vector on the Luc/GFP Reporter cell line is considered minimal. However, we recommend that customers pay attention to the special growth requirements of the NCI-N87 cell line during use, such as culture medium components and culture conditions, to ensure optimal expression and stability of the cells. We provide detailed training guides to help clients overcome potential issues.
A: Yes, our Luc/GFP Reporter Cell Line-NCI-N87 has undergone rigorous contaminant testing, including detection of common contaminants such as mycoplasma and bacteria. We use methods such as PCR and cell culture testing to ensure the purity of our cell lines. Our strict quality control procedures ensure that each batch of cell lines meets high standards of purity.
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During cell culture, this Luc/GFP Reporter Cell Line-NCI-N87 showed amazing stability. The cells remained stable during growth, and there were no fluctuations in gene expression. I used GFP fluorescence and luciferase activity measurement systems for real-time monitoring, and the results were as reliable as ever, with almost no deviation. The supplier's service is also very good, providing detailed instructions and technical support. This gives me great peace of mind when studying gene expression and biological function. All in all, this product has greatly improved my research efficiency.
I have been using this Luc/GFP Reporter Cell Line-NCI-N87 for several months and I am really satisfied with it. The cells grew well in culture, and the gene expression results were very consistent from experiment to experiment. Especially when observing GFP fluorescence, you can clearly see the gene expression inside the cell, which is very intuitive. The supplier's delivery was also very fast and the packaging was very tight without any damage. This cell line makes my research on gastric cancer more convenient and it is highly recommended.
I recently did some experiments with Luc/GFP Reporter Cell Line-NCI-N87, and the results exceeded expectations. The growth status of the cells is very good, and there is almost no contamination. Moreover, when monitoring gene expression in real-time, the signals of GFP and luciferase are very clear, which is very helpful for my data analysis. The after-sales service provided by the supplier is also very timely and professional, allowing me to quickly solve any problems I encountered during use. Very satisfied with this purchasing experience.
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