Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
The Bioluminescent Reporter Cell Lines offered by Creative Biogene cover many cell types, including 4T1, A375, A549, B16F10, and CT26.WT, HCT116, HT1080, LL/2, MCF-7, MDA-MB-231, and SKOV-3. These cell lines are equipped with the luciferase reporter gene, allowing researchers to study various cellular processes and molecular events. With the luciferase reporter, researchers can monitor gene expression, signal transduction, and pathway activation in real time, providing valuable insights into cellular physiology and disease mechanisms. Creative Biogene's Bioluminescent Reporter Cell Lines offer unparalleled versatility and sensitivity, making them indispensable tools for various applications in molecular biology, cell biology, drug discovery, and biomedical research. Leveraging these cell lines enables researchers to accelerate their studies, advance scientific knowledge, and facilitate the development of novel therapeutics.
Advantages of Our Bioluminescent Reporter Cell Lines:
The luciferase reaction takes place within the specialized light organ of fireflies, emitting a yellow-green light at 560nm wavelength. It relies on cofactors such as ATP, Mg2+, O2, and the substrate luciferin. This bioluminescence serves as a valuable tool for measuring luciferase activity, facilitating the monitoring of regulatory elements controlling its expression.
Bioluminescent reporter cell lines utilize the luciferase enzyme, which generates a yellow-green light upon reacting with its substrate luciferin. This light emission, serves as a reliable indicator of luciferase activity and enables the monitoring of regulatory elements controlling gene expression.
Compared to fluorescent proteins, luciferase offers distinct advantages such as high sensitivity, low background interference, and easy quantification. It is particularly suitable for applications requiring precise measurement of gene expression levels and promoter activities. Specific applications of bioluminescent reporter cell lines include:
Case Study 1
The utilization of small interfering RNA (siRNA) holds significant promise in the realm of cancer therapy. Researchers utilized temperature and pH-responsive cationic and amphiphilic pentablock copolymers to develop polyplex and gold nanoparticle-based multicomponent siRNA delivery systems. These copolymers protected siRNA, facilitated cellular entry, and promoted endosomal escape. The study aimed to enhance siRNA transfection efficiency while minimizing toxicity. The results demonstrated a 15% improvement in transfection efficiency compared to conventional methods. This approach holds promise for future siRNA delivery applications, leveraging the synergistic advantages of responsive copolymers and gold nanoparticles.
Figure 1. The developed systems with dye-attached polymers were visualized via confocal microscopy by researchers to assess their interaction with SKOV3 cells, which were plated at a density of 2 × 10^5 cells per well in Petri dishes and incubated for 24 hours at 37°C in a 5% CO2 atmosphere. The luciferase-expressing SKOV3 cells, acquired from Creative Biogene, were grown in high glucose Dulbecco's Modified Eagle's Medium. (Metin Uz, et al., 2015)
Case Study 2
Immunotherapy, including checkpoint inhibitors, bi-specific antibodies, and CAR-T-cell therapies, effectively treat multiple cancers. Researchers employed a PBMC humanized mouse model to assess tumor control and cytokine release profiles following treatment with a CD19xCD3 bispecific T-cell engager (BiTE). This model allows evaluation of therapy efficacy and CRS-related safety issues across different PBMC donors. Results demonstrate predictive capabilities for tumor control and cytokine release, highlighting variability among donors and reproducibility across experiments. This model serves as a sensitive and reproducible platform for identifying effective treatments and potential complications in immuno-oncology.
Figure 2. Cytokine release patterns in humanized NSG-MHC-DKO mice treated with CD19xCD3 bispecific antibodies were investigated by researchers to understand donor and dosage dependencies, aiming to inform therapeutic efficacy against human B cell lymphoma. The Raji_Luc cell line, acquired from Creative Biogene, is a clonal lineage expressing Firefly luciferase with an emission peak wavelength of 550 nm. (Yang J, et al., 2023)
Q: How stable are these Bioluminescent reporter cell lines? Is there a risk of cell mutation or inactivation?
A: Our Bioluminescent reporter cell lines undergo extensive stability testing to ensure stable expression within the cell lines. We mitigate the risk of cell mutation or inactivation through regular cell checks and validation, along with stringent cell culture and storage protocols. Furthermore, stability of our cell lines is confirmed through techniques such as PCR and sequencing, ensuring long-term reliability and consistency. Thus, you can confidently utilize our Bioluminescent reporter cell lines in your research, yielding reproducible and reliable results.
Q: What experimental applications are these Bioluminescent reporter cell lines suitable for?
A: Wide-ranging applications including promoter activity assessment, miRNA studies, tumor growth monitoring, drug screening, cell signaling pathway investigation, and in vivo imaging. High sensitivity, low background interference, and ease of quantification support diverse experiments.
Q: How should these Bioluminescent reporter cell lines be best utilized?
A: We provide detailed user manuals and experimental protocols, covering cell culture conditions, luciferase detection methods, and experimental design recommendations. Additionally, our technical support team is readily available to offer assistance and guidance, addressing any issues encountered during experimentation and providing best practice advice. We recommend thoroughly reviewing relevant documentation before experimental design to ensure adherence to guidelines for accurate and reliable results.
Q: What is the signal strength and stability of these Bioluminescent reporter cell lines?
A: Robust signal strength and stable expression verified. Rigorous validation ensures stability and consistency across experimental conditions. Optimized cell culture conditions and detection methods maximize signal strength and stability. Stringent quality control measures ensure signal quality and reliability of results.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0041 | luciferase Reporter Stable Cell Line-HEK293 | Inquiry |
| CSC-RR0049 | Luciferase Reporter Stable Cell Line-MCF-7 | Inquiry |
| CSC-RR0060 | GFP/Luciferase Reporter Stable Cell Line - SK-OV-3 | Inquiry |
| CSC-RR0061 | Luciferase Reporter Stable Cell Line - SK-OV-3 | Inquiry |
| CSC-RR00610 | Luciferase Reporter Cell Line - Farage | Inquiry |
| CSC-RR00619 | GFP/Luc Reporter Cell Line - Capan-1 | Inquiry |
| CSC-RR00620 | GFP/Luc Reporter Cell Line - Farage | Inquiry |
| CSC-RR00622 | Luciferase Reporter Cell Line - WEHI-3 | Inquiry |
| CSC-RR00623 | GFP/Luc Reporter Cell Line - WEHI-3 | Inquiry |
| CSC-RR0075 | RFP/Luciferase Reporter Stable Cell Line - HEK293 | Inquiry |
| CSC-RR00924 | Luciferase Reporter Cell Line - HEK293T | Inquiry |
| CSC-RR00925 | Luciferase Reporter Cell Line - MPC-5 | Inquiry |
| CSC-RR00926 | Luciferase Reporter Cell Line - Mac-T | Inquiry |
| CSC-RR00927 | Luciferase Reporter Cell Line - SW-13 | Inquiry |
| CSC-RR00928 | Luciferase Reporter Cell Line - Kupffer | Inquiry |
Copyright © Creative Biogene. All rights reserved.