Product Details

Creative Biogene's fluorescent reporter cell lines encompass a diverse array of cell types derived from various organisms, including humans, mice, rats, and other model organisms. These cell lines represent innovative biological tools that play pivotal roles across numerous scientific disciplines, including molecular biology, cell biology, cancer research, drug discovery, and developmental biology. Through the stable expression of green fluorescent protein (GFP) or red fluorescent protein (RFP), these cell lines enable real-time visualization and tracking of dynamic cellular processes, protein localization, gene expression dynamics, and other fundamental biological phenomena. They provide researchers with a versatile platform for investigating cell behavior, screening potential drug candidates, dissecting signaling pathways, and modeling various disease states within a wide range of experimental contexts.

With Creative Biogene's fluorescent reporter cell lines, researchers can deepen their understanding of biological mechanisms, explore novel scientific frontiers, and expedite the development of innovative therapeutics for a multitude of diseases.

Advantages of Creative Biogene's Fluorescent Reporter Cell Lines:

• Diverse Cell Types: Encompass a broad range of cell types derived from humans, mice, rats, and other model organisms, offering versatility in experimental applications.
• Innovative Biological Tools: Serve as innovative tools across multiple scientific disciplines, including molecular biology, cell biology, cancer research, drug discovery, and developmental biology.
• Real-time Visualization: Enable real-time visualization and tracking of dynamic cellular processes, protein localization, and gene expression dynamics through stable expression of GFP or RFP.
• Versatile Platform: Provide researchers with a versatile platform for investigating cell behavior, screening potential drug candidates, dissecting signaling pathways, and modeling various disease states.
• Customizable: Offer customization options to tailor cell lines to specific research needs, ensuring flexibility and adaptability to diverse experimental requirements.

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Application

Labeling proteins within living cells is essential for understanding protein dynamics, mobility, and function, contributing significantly to the comprehension of biological processes. While the fusion of proteins with fluorescent proteins like GFP has enabled the monitoring of cellular localization since 1995, it comes with limitations such as altered localization and function due to GFP's size.

Chemical labeling of proteins offers versatility compared to fusion proteins, allowing labeling with various functionalities, particularly fluorescence, using bright organic dyes of diverse colors. Techniques for chemical protein labeling, especially those applicable within living cells, are continually evolving to be fast, selective, and minimally disruptive.

Specific chemical labeling of proteins inside living cells presents challenges, requiring reactions to occur under mild conditions and be highly selective. Specialized techniques exist, from labeling target proteins via entire domains to labeling endogenous proteins. Fluorescent reporter cell lines offer numerous advantages and have diverse applications in basic and applied sciences, including:

• Quantitative Analysis: Enabling precise measurement of gene expression levels and pathway activation.
• Phenotypic Detection: Providing a visible and quantifiable phenotype for diverse analyses.
• Interactions Studies: Investigating interactions between hosts and pathogens.
• Drug Screening: Identifying potential drug candidates efficiently.
• Toxicity Assessment: Evaluating the toxicity of chemical compounds or drugs accurately.
• Biological Imaging: Enabling real-time visualization of biological processes in vivo.

Case Study

Case Study 1

While cancer stem cell (CSC) niches in the tumor microenvironment (TME) are increasingly recognized for their role in solid tumor progression, many molecular players in this regulation remain unknown. Researchers utilized a murine melanoma model to investigate the impact of β3-adrenergic receptor (β3-AR) blockade on CSC regulation within the TME. By employing pharmacological inhibition of β3-AR, they observed a reduction in CSC marker expression and a shift towards differentiated hematopoietic subpopulations. Specifically, the antagonism of β3-AR led to enhanced hematopoiesis and differentiation of mesenchymal stem cells (MSCs) into adipocytes, potentially hindering stemness traits within the TME. These findings suggest that targeting β3-AR may offer therapeutic benefits by modulating the TME to impede melanoma progression.

Figure 1. Researchers investigated the impact of propranolol and SR59230A on B16-F10 tumor growth in C57BL/6 mice. They assessed tumor growth rates, apoptosis, proliferation, and β2- and β3-adrenoreceptor expression to understand the effects of these treatments. The murine B16-F10 melanoma GFP stable cell line was acquired from Creative Biogene (Shirley, NY, USA). (Calvani M, et al., 2020)

Case Study 2

Vessel co-option (VC) stands apart from angiogenesis as tumor cells exhibit growth towards existing blood vessels. Researchers utilized a needle template method to mimic VC, a less explored phenomenon compared to angiogenesis. They developed a VC on chip model, investigating the distance between glioblastoma spheroids and preformed blood vessels. Despite the distance, cancer cells displayed VC-like behavior by growing towards but not penetrating the vessels. This study highlights the potential of the chip model to recapitulate VC in glioblastoma, aiding in better understanding this clinically significant process.

Figure 2. Researchers aim to report cell line behavior, particularly the migration of U87 MG cells from GBM spheroids at varying distances from vasculature, to understand tumor dynamics in vitro. RFP-labeled U87 cells were obtained from Creative Biogene (Shirley, NY, USA). (Jinseung Bae et al., 2023)

Case Study 3

Breast cancer ranks as the most prevalent cancer among women across various ethnicities and stands as a major contributor to cancer-related mortality worldwide. Researchers aimed to develop a 3D material as a substrate for breast cancer cell culture. Composite gels of varying Alginate (A) and Matrigel (M) concentrations were created for stability and biological activity. Human aggressive breast cancer cells (MDA-MB-231) were cultured within. Through morphological characterization and an innovative bioreactor-based invasion assay, the study revealed significant findings in a 50% Alginate, 50% Matrigel gel: distinctive cytoskeletal shapes, invadopodia formation, and migration towards an engineered membrane, offering a novel 3D in vitro model of early metastatic events.

Figure 3. The tumor hydrogel has MDA-MB-231 cells expressing GFP (Green Fluorescent Protein) for easier monitoring over time. This GFP-labeled cell line, obtained from Creative Biogene, helps visualize cellular dynamics in the hydrogel. (Cavo M, et al., 2018)

FAQ