RFP Reporter Stable Cell Line-HEK293

Name: RFP Reporter Stable Cell Line-HEK293
SKU: CSC-RR0066
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0066

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RR0066
Description	This cell line is engineered to stably overexpress the RFP in HEK293 cells.
Introduction	Red Fluorescent Protein (RFP) is a genetic mutant of green fluorescent protein.
Background	The development of the RFP Reporter Stable Cell Line-HEK293 traces its origins to the early 1970s with the discovery and establishment of the Human Embryonic Kidney 293 (HEK293) cell line by Graham et al. in 1977. Initially derived from human embryonic kidney cells
Target Gene	RFP
Abbr	HEK293-RFP
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Reporter Type	Fluorescent protein
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Case Study

Applications

Q & A

Customer Reviews

Posttranslational modifications extensively alter the ubiquitous transcription factor, specificity protein 1 (SP1). Researchers employ RFP Reporter Stable Cell Line-HEK293 to probe SP1's role in Wnt signaling. This cell line facilitates studying SP1's stability modulation by Wnt components like GSK3β, β-TrCP E3 ubiquitin ligase, and β-catenin. Monitoring RFP reporter expression allows dynamic assessment of SP1 degradation upon Wnt activation, unveiling SP1-β-catenin crosstalk's impact on target gene expression. This study unveils pivotal insights into SP1-β-catenin interplay in Wnt signaling.

Figure 1. Researchers discovered that RFP SP1 is vital for stabilizing β-catenin and regulating Wnt-responsive genes. Immunofluorescence analysis in siSP1-transfected HCT116 cells showed reduced nuclear SP1 and β-catenin. Immunoblots in Wnt3A-treated HEK293 cells upon SP1 depletion exhibited altered SP1, β-catenin, and TCF7 levels. (Mir R, et al., 2018)

1. Fluorescence Imaging: Visualize protein localization dynamics in live cells.

2. Drug Screening: Assess efficacy and toxicity of compounds on target proteins.

3. Gene Expression Analysis: Monitor promoter activity or gene regulation in response to stimuli.

4. Protein-Protein Interaction Studies: Investigate protein interactions via Förster resonance energy transfer (FRET) assays.

5. Cell Signaling Pathway Analysis: Study signal transduction pathways by observing changes in fluorescence intensity upon pathway activation.

6. High-Throughput Screening: Screen libraries of compounds for their effects on cellular processes.

Customer Q&As

Why were HEK293 cells chosen for establishing the RFP reporter stable cell line?

A: HEK293 cells were chosen for establishing the RFP reporter stable cell line due to their similar advantages to the GFP reporter line, including high transfection efficiency and suitability for protein expression studies. Additionally, RFP provides an alternative fluorescence marker for multiplexing experiments.

How was the stability and expression level of RFP verified and sustained in the HEK293 stable cell line?

A: The stability and expression level of RFP in the HEK293 stable cell line was verified and sustained through stable transfection and antibiotic selection. Expression was validated using fluorescence microscopy or flow cytometry, and stability was ensured through regular culture maintenance and monitoring of fluorescence intensity.

What insights can you share regarding the functional characterization of RFP expression in the HEK293 stable cell line, focusing on its responsiveness to regulatory elements and cellular processes?

A: Insights into the functional characterization of RFP expression in the HEK293 stable cell line included assessing its responsiveness to regulatory elements through changes in fluorescence intensity in response to stimuli. Additionally, RFP's involvement in cellular processes such as protein trafficking or organelle labeling was investigated using live-cell imaging or co-localization studies.

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Customer Reviews

Consistent and bright expression

Consistent expression! This cell line surpasses expectations, providing bright and uniform RFP fluorescence, facilitating accurate quantification and visualization of cellular processes. Its stable RFP expression simplifies experimental workflows, allowing for rapid data acquisition and analysis, and accelerating discoveries in cell biology and molecular biology.

United States

2023-04-16

Notable RFP fluorescence

Remarkable fluorescence! The RFP Reporter Stable Cell Line in HEK293 cells offers stable expression of RFP, enabling easy visualization and tracking of cellular events in my research.

United States

2022-09-26

Valuable tool for visualizing cellular events

The RFP Reporter Stable Cell Line has significantly enhanced my research capabilities, offering a versatile platform for studying gene expression dynamics and protein localization in vitro.

French

2024-04-18

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RFP Reporter Stable Cell Line-HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Case Study

Applications

Q & A

Customer Reviews

Why were HEK293 cells chosen for establishing the RFP reporter stable cell line?

How was the stability and expression level of RFP verified and sustained in the HEK293 stable cell line?

What insights can you share regarding the functional characterization of RFP expression in the HEK293 stable cell line, focusing on its responsiveness to regulatory elements and cellular processes?

Consistent and bright expression

Notable RFP fluorescence

Valuable tool for visualizing cellular events

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