RFP Reporter Stable Cell Line-HEK293

Name: RFP Reporter Stable Cell Line-HEK293
SKU: CSC-RR0066

For research use only. Not intended for any clinical use.

Cat.No.

CSC-RR0066

Description

This cell line is engineered to stably overexpress the RFP in HEK293 cells.

Background

The development of the RFP Reporter Stable Cell Line-HEK293 traces its origins to the early 1970s with the discovery and establishment of the Human Embryonic Kidney 293 (HEK293) cell line by Graham et al. in 1977. Initially derived from human embryonic kidney cells

Abbr

HEK293-RFP

Growth Properties

Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Host Cell

HEK293

Introduction

Red Fluorescent Protein (RFP) is a genetic mutant of green fluorescent protein.

Morphology

Epithelial

Quality Control

Negative for bacteria, yeast, fungi and mycoplasma.

Shipping

Dry ice

Stability

Validated for at least 10 passages

Storage

Liquid nitrogen

Applications

Publications

Q & A

Customer Reviews

Fluorescence Imaging: Visualize protein localization dynamics in live cells.

Drug Screening: Assess efficacy and toxicity of compounds on target proteins.

Gene Expression Analysis: Monitor promoter activity or gene regulation in response to stimuli.

Protein-Protein Interaction Studies: Investigate protein interactions via Förster resonance energy transfer (FRET) assays.

Cell Signaling Pathway Analysis: Study signal transduction pathways by observing changes in fluorescence intensity upon pathway activation.

High-Throughput Screening: Screen libraries of compounds for their effects on cellular processes.

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