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Adenoviral vectors are a multifunctional research tool with several positive aspects. The recombinant adenovirus can infect dividing and non-dividing cells, support transient, high-level protein expression, and are also easily amplified and purified to high concentrations. Under normal circumstances, they can accommodate inserts of up to 8 kb. Larger inserts are possible, in case an equivalent part of the viral genome has been properly deleted. The Viral Vector Core (VVC) is equipped to produce adenoviral vectors with inserts up to 10kb.
As a leader in adenoviral technology, Creative Biogene has developed a comprehensive library of human, mouse, and rat genes cloned into adenoviral vectors or ready-to-use adenoviruses, which can be used to manipulate the expression of your gene of interest within a wide range of host cells. Creative Biogene offers many different kinds of premade adenoviruses containing full-length ORFs of particular genes or shRNA/miRNA with a choice of different tags or reporter genes as you want.
Creative Biogene provides premade adenovirus particles expressing reporter genes including GFP, RFP, luciferase etc.
Creative Biogene has produced a list of prepackaged adenoviruses that are suitable for control use.
Creative Biogene offers adenovirus particles expressing cell reprogramming factors for development of iPS cells.
Creative Biogene is offering prepackaged adenoviruses containing cre recombinase that can be used in genome editing.
Creative Biogene provides adenoviral particles containing various ORFs that can be used for transient gene expression.
Creative Biogene offers adenovirus particles to support miRNA research.
Creative Biogene offers pre-made tumor-specific, replication-competent oncolytic adenoviruses to support the investigation of oncolytic immunotherapy.
Adenoviruses are non-enveloped icosahedral particles with linear double-stranded DNA genomes. Recombinant adenoviral vectors can efficiently transduce both dividing and non-dividing mammalian cells, enabling high-level transient transgene expression without genomic integration. Owing to their strong infectivity, rapid expression, large capacity of up to 10 kb, and scalable production to high titers, adenoviral vectors have become prevalent tools for genetic manipulation in basic research, vaccine development, and gene therapy investigations.
You can use the adenovirus products we provide to achieve the following types of molecular biology experimental applications:
Case Study 1
Coenzyme Q1 (DUOXA1) is implicated in the maturation of dual oxidase 1 (DUOX1), an enzyme generating reactive oxygen species (ROS) in the adult thyroid. However, ROS is also associated with the development of various tissues. To ascertain whether DUOXA1 levels impact muscle differentiation, an adenovirus construct (pCMV5-DUOXA1-GFP) was employed to drive constitutive overexpression of this protein in primary myoblasts. Researchers reveal robust expression of DUOXA1 in activated muscle satellite cells and primary myoblasts isolated from mice, with levels changing during cell differentiation, demonstrating the significance of DUOXA1 in skeletal muscle myoblasts.
Figure 1. DUOXA1 overexpression in primary myoblasts inhibits differentiation. Primary myoblasts were infected with adenoviral vectors containing pCMV5-GFP (GFP), or pCMV5-DUOXA1-GFP (GFP-DUOXA1). (Sandiford, S D, et al., 2014)
Case Study 2
Delta-24-RGD, an experimental adenovirus-based therapy for cancer, was investigated for its ability to induce autophagy in pancreatic cancer cells. Cells were treated with PBS or Delta-24-RGD virus (MOI of 1) for 3-5 days, followed by the addition of 10μl LC3B-GFP virus and a 24-hour incubation. Fluorescence microscopy revealed LC3-GFP-positive autophagosomes. Ad-GFP-RGD (Ad-GFP) or Delta-24-RGD was used as a control, and protein expression levels were determined through immunoblotting of cell lysates with specific antibodies. Results confirm that Delta-24-RGD induces autophagy in pancreatic cancer cells.
Figure 2. D-24-RGD induces pancreatic cancer cell autophagy, evident in increased LC3B-GFP puncta after 5 days of infection. Quantification revealed a significant rise. Acridine orange staining demonstrated elevated acidic vesicle organelles after 5 days of D-24-RGD infection at various MOIs. (Dai, B. et al., 2017)
Q: What is adenovirus?
A: Adenovirus is a double-stranded DNA virus that can infect humans and a variety of animals, causing respiratory and digestive tract infections.
Q: Under what circumstances should an adenoviral vector be chosen?
A: Adenovirus is usually chosen as a gene delivery vector under the following circumstances:
(1) When the target gene to be transduced is large. Adenovirus has a larger capacity and can accommodate larger foreign genes.
(2) When the experiment needs to be completed in a short period and the cycle is tight. Adenovirus can rapidly amplify 36-48 hours after infection.
(3) When the target cells are difficult to infect. Adenovirus has a wide adaptability and can infect a variety of cells.
(4) When there is no need to establish stable expression cell lines. Adenovirus infection is transient.
(5) When animal experiments are required. Adenoviruses can infect a variety of animal cells.
Q: What are the packaging systems for adenovirus?
A: Commonly used packaging systems for adenovirus include 293 cells, PER.C6 cells, etc. Stable expression of adenovirus structural proteins in these cells can provide structural proteins required for virus assembly.
Q: What experimental steps are required to infect cells with adenovirus?
A: The main steps of adenovirus infection of cells:
(1) Viruses adsorb to cell surface receptors
(2) Viruses enter cells
(3) Viral DNA enters the cell nucleus
(4) Viral gene expression
(5) Viral DNA replication
(6) Virus assembly
(7) Virus Release
Q: How are adenoviral vectors constructed?
A: The main steps in constructing adenoviral vectors are:
(1) Construct a transposable vector: insert the target gene, promoter, etc. into the transposable plasmid
(2) Preparation of viral genomic DNA
(3) Co-transfect the transposable vector and genomic DNA into packaging cells
(4) Amplification of recombinant adenovirus
| Cat.No. | Product Name | Price |
|---|---|---|
| ONCOADV-001 | Ad5-hTERT-E1A-E1B[GFP] Oncolytic Adenovirus | Inquiry |
| ONCOADV-002 | Ad5/RGD-hTERT-E1A-E1B[GFP] Oncolytic Adenovirus | Inquiry |
| ONCOADV-003 | Ad5-E1A(D24)-E1B[GFP] Oncolytic Adenovirus | Inquiry |
| ONCOADV-004 | Ad5/RGD-E1A(D24)-E1B[GFP] Oncolytic Adenovirus | Inquiry |
| ONCOADV-005 | Ad5-hTERT-E1A-E1B-mCMV-mGM-CSF Oncolytic Adenovirus | Inquiry |
| AD00007Z | LacZ adenoviral particles | Inquiry |
| AD00036Z | Human OCT4 adenoviral particles | Inquiry |
| AD00037Z | Human Sox2 adenoviral particles | Inquiry |
| AD00038Z | Human myc adenoviral particles | Inquiry |
| AD00040Z | Human OCT4,Sox2,KLF4 adenoviral particles | Inquiry |
| AD00043Z | Human E2F1 adenoviral particles | Inquiry |
| AD00044Z | Human hTERT adenoviral particles | Inquiry |
| AD00045Z | Human GPR6 adenoviral particles | Inquiry |
| AD00046Z | Human DRD1 adenoviral particles | Inquiry |
| AD00047Z | Human DRD2 adenoviral particles | Inquiry |
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