Cat.No. : AD00007Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00007Z |
| Target Gene | lacZ |
| Product Type | Adenoviral particle |
| Insert | LacZ |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
| Gene Name | lacZ beta-D-galactosidase [ Cronobacter turicensis z3032 ] |
| Gene Symbol | LacZ |
| Synonyms | LACZ; beta-D-galactosidase; EC 3.2.1.23; |
| Gene ID | 8460891 |
| Pathway | Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Other glycan degradation, organism-specific biosystem; Other glycan degradation, conserved biosystem; Sphingolipid metabolism, organism-specific biosystem; Sphingolipid metabolism, conserved biosystem; |
Angiopoietin-like protein 2 (ANGPTL2) is a recently discovered proinflammatory cytokine that is primarily secreted by adipose tissue. Here, researchers delivered adenovirus-mediated lacZ (Ad-LacZ) or human ANGPTL2 (Ad-ANGPTL2) to diabetic db/db mice via the tail vein. Ad-ANGPTL2 treatment for 2 weeks impaired glucose tolerance and insulin sensitivity compared with Ad-LacZ treatment. Ad-ANGPTL2 treatment significantly induced proinflammatory gene expression in white adipose tissue. Ad-ANGPTL2-treated mice had increased adipose tissue macrophages (F4/80+CD11b+) and an increased M1 macrophage subset (F4/80+CD11b+CD11c+). In addition, Ad-ANGPTL2 treatment increased the CD8-positive T cell population in adipose tissue, which preceded the increase in macrophage accumulation. Consistent with the in vivo results, recombinant human ANGPTL2 protein treatment increased the mRNA levels of pro-inflammatory gene products and TNF-α protein production in the human macrophage-like cell line THP-1. In addition, Ad-ANGPTL2 treatment induced lipid accumulation in the liver of mice and increased the expression of genes related to fatty acid synthesis and lipid metabolism. These results suggest a potential mechanism for insulin resistance.
Although there was no difference in liver weight between Ad-ANGPTL2 and Ad-LacZ, the livers of Ad-ANGPTL2-treated mice appeared to be paler than those of Ad-LacZ-treated mice (Figure 1A). Ad-ANGPTL2 increased hepatic triglyceride content in db/db mice compared with Ad-LacZ (Figure 1B). Oil Red O staining showed that livers of Ad-ANGPTL2-treated mice tended to have larger staining areas (Figures 1C and 1D). Gene expression analysis was assessed in the liver. Human ANGPTL2 gene expression was significantly induced (Figures 1E and 1F). Ad-ANGPTL2 treatment increased the expression of genes related to fatty acid synthesis and metabolism (PPARα, Acacb, Acly, and Fasn) in lean mice and db/db mice (Figures 1E and 1F). Although there was no significant difference in the results of Oil Red O staining, Ad-ANGPTL2 treatment increased the synthesis of fatty acids, the expression of metabolism-related genes, and the accumulation of lipids in the liver, and may induce fatty liver in mice.
Figure 1. ANGPTL2 enhanced hepatic lipid accumulation in mice. (Sasaki Y, et al., 2015)
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The LacZ adenoviral particles offered by Creative Biogene have become a staple in our lab due to their reliability and consistency. These particles are perfect for monitoring gene expression, providing clear and accurate outcomes every time.
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