Ad5-E1A(D24)-E1B[GFP] Oncolytic Adenovirus

Name: Ad5-E1A(D24)-E1B[GFP] Oncolytic Adenovirus
SKU: ONCOADV-003

Cat.No. : ONCOADV-003

Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL

Storage: -80℃ Shipping: Frozen on dry ice

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Adenovirus Particle Information

Quality Control

Cat. No.	ONCOADV-003
Description	This product is a type of oncolytic adenovirus (OAd) that is derived from human adenovirus serotype 5 (hAd5). This OAd contains E1A and E1B in which a 24 bp in the conserved region 2 (CR2) of E1A is deleted. It also contains GFP reporter gene expression cassette, and the E3 region in this OAd is completely deleted. The CR2 (Delta-24) mutation enables this OAd to infect tumor cells with mutations in the Rb/p16 pathway and lead to cell lysis.
Background	The use of oncolytic adenoviruses (OAds) is a promising approach in cancer therapy as they can be engineered to specifically replicate in tumor cells, while leaving healthy cells unharmed. The replication of OAds in tumor cells eventually leads to lysis and death of tumor cells. Additionally, they can be combined with other treatments, such as chemotherapy or radiation therapy, to enhance their effectiveness. Clinical trials have shown some success in using oncolytic adenoviruses to treat various types of cancer, including head and neck cancer, melanoma, and bladder cancer etc.
Reporter	GFP
Serotype	Human adenovirus serotype 5 (hAd5)
Product Type	Oncolytic adenovirus particles
Applications	Investigation of oncolytic virotherapy for the treatment of cancer using oncolytic adenoviruses
Warning	Please handle this product under Biological Safety Manual (BSL-2) facilities.
Titer	Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc.
Size	Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance.
Sterility	Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination.
Ad5 E1 Detection	All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination.
RCA Assays	Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources.
PFU Titering	All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells.

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