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Human TLR4/NF-kB/SEAP Reporter Stable Cell Line-HEK293

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Cat.No.
CSC-RR0004
Background
TLR4 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. Placental alkaline phosphatase is one of the most stable isoenzyme, only existing in the placenta of higher primates. These characteristics make placental alkaline phosphatase suitable to use as a reporter gene for the analysis of promoter activity and gene expression in cell culture and animal serum. The natural form of placental alkaline phosphatase (PLAP) is membrane anchored. The recombinant form of placental alkaline phosphatase (secreted alkaline phosphatase, SEAP) is used for reporter gene function. SEAP is created by inserting a translational terminator after amino acid 489 (Berger, et al., Gene 66 : 10. This mutation converts the membrane-bound PLAP protein into the secreted protein. As a major transcription factor, NF-kB plays a key role in regulating genes responsible for the innate and adaptive immune responses. In unstimulated cells, the NF-kB dimers are held in the cytoplasm by IkBs that masks the nuclear localization signals of NF-kB. Upon cell stimulation, which leads to IkB degradation, NF-kB quickly translocates to the nucleus and activates various genes that have DNA-binding sites for NF-kB.
Growth Properties
Adherent
Morphology
Epithelial
Host Cell
HEK293
Ship
Dry ice
Gene Information
Official Symbol
TLR4
Synonyms
TLR4; toll-like receptor 4; CD284; hToll; TLR 4; homolog of Drosophila toll; TOLL; TLR-4; ARMD10;
Gene ID
UniProt ID
O00206
Chromosome Location
9q33.1
Pathway
Activated TLR4 signalling, organism-specific biosystem; Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; IKK complex recruitment mediated by RIP1, organism-specific biosystem;
Function
lipopolysaccharide binding; lipopolysaccharide binding; lipopolysaccharide receptor activity; phosphatidylinositol 3-kinase binding; protein binding; receptor activity; transmembrane signaling receptor activity;

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