Cat.No. : AAV00026Z
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV Serotype 2 Storage: -80 ℃
| Cat. No. | AAV00026Z |
| Description | LacZ Adeno-associated virus(AAV Serotype 2) which expresses β-galactosidase (LacZ) under CMV promoter. LacZ is frequently used as reporter gene. This is achieved by staining the tissues or cells with substrates like X-gal, which produces a blue color in the presence of beta-galactosidase. |
| Reporter | LacZ |
| Serotype | AAV Serotype 2 |
| Product Type | Adeno-associated virus |
| Application | 1. Determination of optimal MOI (multiplicity of infection), administration methods etc. 2. Detection of the infection efficiency of the AAV serotype against a specific cell type or tissue. 3. Using reporter genes to visualize the distribution and expression of AAV vectors in live animals, helping assess the biodistribution and persistence of gene delivery. |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
| Gene Name | lacZ beta-D-galactosidase [ Cronobacter turicensis z3032 ] |
| Gene Symbol | LacZ |
| Synonyms | LACZ; beta-D-galactosidase; EC 3.2.1.23; |
| Gene ID | 8460891 |
| Pathway | Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Other glycan degradation, organism-specific biosystem; Other glycan degradation, conserved biosystem; Sphingolipid metabolism, organism-specific biosystem; Sphingolipid metabolism, conserved biosystem; |
Transplantation of cells into the infarcted heart has the potential to improve myocardial recovery significantly. However, the low efficiency of cell implantation still limits the therapeutic efficacy. Here, researchers report the results of competitive screening of most known chemokines and interleukins to assess their ability to promote cardiac engraftment of bone marrow (BM)-derived MSCs and to rank their relative efficacy. This competitive implant screen was performed by adapting the in vivo functional selection (FunSel) method. FunSel is based on the use of a panel of secreted factors, each encoded by a different adeno-associated virus (AAV) vector. Here, mixed preparations of these vectors were used to transduce MSCs in vitro and then transplant them into the heart under normal and ischemic conditions. By ranking 80 different cytokines and growth factors for their ability to stimulate heart engraftment by mesenchymal stromal cells, cardiotrophin-1 (Ctf1) was found to be the most potent factor in both normal and infarcted hearts. Enhanced engraftment of Ctf1-preconditioned mesenchymal stromal cells was mediated by the rapid activation of the focal adhesion complexes of the cells.
Proper adhesion to the extracellular matrix is known to be a major determinant of cell survival in many experimental settings and appears to be particularly important following cell transplantation in environments of hypoxia and high oxidative stress levels, such as the infarcted myocardium. Indeed, the researchers found that Ctf1 protected BM-MSCs from oxidative damage. Cells were treated with hydrogen peroxide after transduction with AAV2-Ctf1 or pretreated with rCtf1. In both cases, the number of apoptotic cells (quantified by terminal deoxynucleotidyl transferase dUTP nick end labeling positivity) was reduced by more than 2.5-fold compared to the corresponding controls (transduction with AAV2-LacZ or untreated cells, respectively; Figures 1A and 1B are representative images and quantification, respectively). Caspase 3/7 activation was also measured in cells treated with rCtf1 and this apoptotic marker was similarly reduced (Figure 1C).
Figure 1. Cardiotrophin-1 (Ctf1) protects bone marrow–derived mesenchymal stromal cells (BM-MSCs) from apoptosis. (Bortolotti F, et al., 2017)
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