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Cre RSV GFP adenoviral particles

Cre RSV GFP adenoviral particles

Cat.No. :  AD00300Z

Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL

Storage:  -80℃ Shipping:  Frozen on dry ice

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Adenovirus Particle Information

Quality Control

Gene Informationn

Cat. No. AD00300Z
Description Cre RSV GFP adenoviral particle expresses Cre via a CMV promoter in the E1 region of the virus and expresses GFP from an RSV promoter in the E3 region. Ready-made control adenoviruses (Ad) are generally in stock and can ship anytime. These particles are CsCl purified and supplied in 100 uL aliquots at 1X10^12 pts/mL. These preps are RCA free and PFU titered.
Target Gene cre
Product Type Adenoviral particle
Caution Virus is supplied at 1X10^12 particles/mL. We recommend thawing the virus at room temperature. Do not make aliquots in PCR tubes or eppendorf centrifuge tubes. We do not recommend diluting the virus and refreezing unless you use the cryo-protective buffer
Insert Cre
Titer Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc.
Size Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots.
Endotoxin Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance.
Sterility Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination.
Ad5 E1 Detection All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination.
RCA Assays Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources.
PFU Titering All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells.
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