Ad5/F35-Cre Chimeric Adenovirus

The Ad5/F35-Cre chimeric adenovirus is a genetically engineered viral vector designed to efficiently deliver Cre recombinase into CAR-negative human cell populations. This chimeric vector integrates the fibrospheres and axons of adenovirus type 35, enabling it to enter cells using the universally expressed CD46 receptor. This modification is crucial for transducing hematopoietic stem cells, mature lymphocytes, and certain cancers resistant to standard Ad5 infection. The vector is non-replicating and expresses Cre recombinase, which specifically recognizes and mediates recombination of loxP DNA sequences. Driven by a constitutive promoter, this system ensures stable Cre expression, enabling irreversible modification of loxP DNA targets without host genome integration or permanent viral replication.

The application of the Ad5/F35-Cre chimeric adenovirus is crucial for complex genome editing and functional genomics studies in human hematology and CAR-deficient tumor models. Researchers utilize this vector to activate or inactivate specific genes in primary T cells, B cells, and monocytes, enabling in-depth studies of immune signaling and cell differentiation. In cancer research, it is an important tool for inducing Cre-mediated recombination in CAR-negative tumor cells, helping to study oncogenic pathways and validate therapeutic targets in a human-relevant context. The Ad5/F35-Cre system is also widely used in lineage tracing experiments and for constructing conditional gene knockout models in hematopoietic lineages. By providing a reliable method for delivering high concentrations of Cre recombinase to historically “hard-to-handle” cells, this chimeric adenovirus facilitates rapid and precise manipulation of the human genome, enabling applications in basic science and translational research.