pComb3HTT vector

The pComb3HTT vector is a robust molecular tool with a myriad of applications in biotechnology and biomedical research specifically in phage display and bacterial expression of recombinant antibodies or antibody fragments. It is noted for its possession of a gene that engages in the expression of human Fab or Fragment antigen-binding to tetanus toxin, which typically serves as a control element for the expression of the Fab. One of the principal applications of the pComb3HTT vector is in the field of phage display technology. Phage display is a molecular biology technique that utilizes bacteriophages (viruses that infect bacteria) to create large libraries of peptide sequences or full-length proteins for use in drug discovery and development. Here, the pComb3HTT vector is primarily used in generating antibody phage libraries from which novel antibodies or antibody fragments can be selected. For soluble Fab expression, the pComb3HTT vector can be conveniently manipulated. The gene coding for the pIII phage fusion protein can be removed by utilizing a specific SpeI/NheI digest process, allowing for the expression of soluble Fragment antigen-binding (Fab). This technique is beneficial for producing antibodies or antibody fragments for biochemical and biophysical studies, therapeutic applications, and diagnostic purposes.