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Reporter stable cell lines serve as invaluable tools for scientific research, facilitating the expression of reporter proteins engineered through the integration of reporter expression cassettes into cell genomes using plasmid or lentivirus vectors. Commonly utilized in life science and biotechnology studies, these reporter genes, such as green fluorescent protein (GFP), red fluorescent protein (RFP), luciferase, and secreted alkaline phosphatase (SEAP), offer visual identification.
These reporter cell lines are categorized based on the upstream regulator of the reporter gene into three groups: Constitutive Reporter Cell Lines, maintaining stable expression under a strong promoter like CMV; Inducible Reporter Cell Lines, enabling controlled expression in response to specific stimuli via inducer response elements; and Signaling Pathway-Specific Reporter Cell Lines, tailored to monitor the activity of specific signaling pathways by regulating reporter gene expression based on pathway activation.
Drawing upon years of experience and thorough investigation, scientists at Creative Biogene have successfully developed numerous stable reporter cells across various popular cell lines, catering to diverse research applications.
Advantages of Our Reporter Cell Lines
Creative Biogene's Constitutive Reporter Cell Lines provide stable expression of fluorescent proteins or luciferase for ongoing cellular process monitoring.
Creative Biogene's Signaling Pathway Reporter Cell Lines enable precise real-time monitoring and analysis of cellular signaling cascades, facilitating efficient exploration of complex cellular pathways.
Creative Biogene's Inducible Reporter Cell Lines enable dynamic pathway activation tracking via controlled reporter gene expression, facilitating precise cellular response investigations.
Originating from the need to study gene expression and cellular processes, Reporter Cell Lines are engineered cell models that express reporter genes, facilitating the monitoring of various cellular activities. They are categorized based on the type of reporter gene they harbor, such as luciferase, fluorescent proteins, or β-galactosidase. These cell lines find applications across diverse research areas, including gene expression analysis, drug screening, pathway elucidation, toxicity testing, and disease modeling. Reporter stable cell lines facilitate:
Case Study 1
The complexity of manufacturing autologous chimeric antigen receptor (CAR) T cell therapies often leads to treatment delays or exclusion of patients. Researchers developed universal allogeneic CAR T cells, using CRISPR-Cas9 to create hypoimmune (HIP) T cells with disrupted B2M, CIITA, and TRAC genes. Lentiviral transduction expressed CD47 and anti-CD19 CAR. Comparative assays showed similar performance between HIP and allo CAR T cells in cancer killing. In mice, HIP CAR T cells cleared tumors as effectively as allo CAR T cells, particularly in fully immunocompetent humanized mice. Targeted CD47 safety strategies reliably eliminated HIP CAR T cells. These findings suggest universal allogeneic HIP CAR T cell therapies could offer durable anti-tumor responses by overcoming limited persistence.
Figure 1. Researchers repeatedly challenged CAR T cells with Nalm6 tumor cells in vivo to assess their efficacy in combating tumor growth and to evaluate their persistence in the spleen and bone marrow. The Nalm6 fluc+ cells were obtained from Creative Biogene (Shirly, NY, cat.no. CSC-RR0361). (Hu X, et al., 2023)
Case Study 2
Temozolomide (TMZ) adjuvant chemotherapy is a fundamental component of glioblastoma multiforme (GBM) treatment, designed to eradicate remaining GBM cells. Researchers investigated the impact of TMZ chemotherapy, often used in glioblastoma multiforme (GBM) therapy, on brain extracellular matrix (ECM). They aimed to understand how chemotherapy affects GBM relapse. By studying an advanced GBM animal model, they found that TMZ, especially when combined with dexamethasone (DXM), altered brain ECM, promoting GBM cell adhesion, proliferation, and invasion. This suggests that changes in ECM composition caused by chemotherapy contribute to GBM relapse. These findings highlight the potential of ECM-targeted therapy to improve GBM patient outcomes by mitigating off-target chemotherapy effects.
Figure 2. Researchers aim to assess TMZ and/or DXM effects on GBM cell adhesion, proliferation, and invasion in brain slices ex vivo, employing confocal microscopy and quantitative analysis for validation. The U87-RFP cell line was acquired from Creative Biogene located in Shirley, NY, USA. (Tsidulko AY, et al., 2021)
Q: What is the stability of the reporter cell lines? Are there risks of cell mutation or inactivation?
A: Our reporter cell lines undergo extensive stability testing to ensure consistent expression of reporter proteins. Additionally, regular cell checks and validation are performed to maintain cellular health and stability. We adhere to strict cell culture and preservation protocols to minimize the risk of cell mutation or inactivation. Thus, researchers can confidently utilize our products for studies, yielding consistent and reliable results.
Q: What experimental applications are suitable for these reporter cell lines?
A: Creative Biogene's reporter cell lines are extensively applicable across various experimental scenarios. They can be employed for gene expression and regulation analyses, drug screening, investigations into cellular signaling pathways, and studies on tumor development, among other fields. These cell lines support both quantitative and qualitative experiments, facilitating techniques such as fluorescence microscopy, live-cell imaging, flow cytometry, and immunofluorescence staining.
Q: Are there any recommendations on the optimal use of these reporter cell lines?
A: We provide comprehensive user manuals and experimental protocols, detailing cell culture conditions, methods for detecting reporter proteins, and experimental design suggestions. Furthermore, our technical support team is available to offer assistance and guidance, addressing any issues encountered during experiments and providing best practice recommendations. We are committed to ensuring researchers can fully utilize our products to achieve successful research outcomes.
Q: How to select the appropriate reporter cell lines for one's research needs?
A: Choosing the right reporter cell lines is crucial for successful research outcomes. Here are some suggestions for selecting the appropriate reporter cell lines for your research needs:
(1) Identify research objectives: Determine the purpose of your study. Whether it's investigating gene expression, cellular signaling pathways, drug screening, or other aspects, this will help identify the required types and characteristics of reporter proteins.
(2) Consider experimental techniques: Take into account the experimental techniques you plan to use. For instance, if you intend to conduct fluorescence microscopy observations, cell lines expressing fluorescent proteins may be more suitable. For quantitative analysis, cell lines expressing proteins like luciferase or SEAP, which can be quantitatively detected, may be preferred.
(3) Select cell types: Determine the cell types you need. Reporter cell lines are available in various cell types such as HEK293, HeLa, CHO, MDCK, etc. Selecting the appropriate cell type for your research object is crucial.
(4) Consider signaling pathways: If you're studying specific signaling pathways such as NF-κB or AP-1, selecting corresponding reporter cell lines may be more targeted and efficient.
By carefully considering these factors and selecting the appropriate reporter cell lines based on your research needs, you can ensure successful research outcomes and reliable results.
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