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MSCV-based Retrovirus Service

MMLV retrovirus has been broadly used for gene transduction in a variety of cells. However, expression of genes mediated by MMLV retrovirus can be repressed in many cell types including embryonic stem (ES), embryonal carcinoma (EC) and hematopoietic stem (HS) cells. The transcriptional repression is mainly caused by DNA methylation.

Retroviral silencers identified in the MoMLV LTR promoter region. Trans‐acting factors (indicated in Green) directly recognize specific DNA sequences or DNA methylation (lollipops). NCR: Negative control region, PBS: Primer binding site. Figure 1. Retroviral silencers identified in the MoMLV LTR promoter region. Trans‐acting factors (indicated in Green) directly recognize specific DNA sequences or DNA methylation (lollipops). NCR: Negative control region, PBS: Primer binding site. (Akitsu Hotta and James Ellis, 2008, J Cell Biochem)

Recombinant MSCV (Murine stem cell virus) retroviral vectors are appealing vehicles for introducing and expressing of target genes into a larger host range including ES, EC and HS cells. The long terminal repeat (LTR) region of MSCV retroviral vectors is different from that of MMLV retroviral vectors. The MSCV LTR is derived from the murine stem cell PCMV virus and contains point mutations and deletions to prevent transcriptional suppression. Thus, the MSCV-based retroviral vectors is more effective than MMLV-based vectors.

Schematic diagram for MSCV retrovirus production Figure 2. Schematic diagram for MSCV retrovirus production

Creative Biogene is offering MSCV-based retrovirus production services that can assist your gene transduction into a variety of cells including ES and EC cells. Scientists at Creative Biogene have developed a proprietary platform which allows us to provide our customers with MSCV retroviruses in high titer, purity and consistency. Our MSCV system includes an optimized packaging cell line and custom construction of transfer vector to meet various requirement from our customers.

Applications

-Gene delivery into a broad range of cell types including embryonic stem cells and embryonic carcinoma cells
-iPS cell line generation
-In vivo animal tests

Features

-One-stop solution: from vector design, to DNA synthesis, to MSCV-based retrovirus production
-Produce virus in high titer that can be used for in vivo injection
-Rigorous quality controls

References:

  1. Lijian S, Wen SW, Gene-delivery systems for iPS cell generation. Expert Opin Biol Ther. 2010 Feb; 10(2): 231–242.
  2. Hotta A, Ellis J. Retroviral vector silencing during iPS cell induction: An epigenetic beacon that signals distinct pluripotent states. J Cell Biochem. 2008;105(4):940–948.
For research use only. Not intended for any clinical use.