Cas9 Stable Cell Line - U2OS

DNA double-strand breaks (DSBs) are the most severe type of DNA damage, causing chromosomal translocations, genomic instability, and cell death. The researchers created the CDDR (CRISPR-Cas9-based Dual-fluorescent DSB Repair) assay to precisely detect and quantify DSB repair outcomes in mammalian cells. CDDR can discriminate between high-fidelity (HF), error-prone non-homologous end-joining (NHEJ), and homology-directed repair (HDR) by inserting one or two DSBs into an intrachromosomal fluorescent reporter with Cas9 and sgRNAs. CDDR demonstrated that HF-NHEJ is dependent on DNA Ligase IV, XRCC4, and XLF and that deleting these genes promotes HDR and error-prone end-joining. The deletion of the ATM kinase boosted HF-NHEJ and decreased HDR, demonstrating CDDR's value in researching DSB repair mechanisms.

Figure 1. Using the CDDR reporter assay, the researchers identify and assess DSB repair. Cas9-sgRNA-expressing plasmid transfections in U2OS-CDDR cells are followed by FACS analysis to determine Mut-NHEJ repair efficiency. (Eki R, et al., 2023)

Enhance your genome editing research with Creative Biogene's Cas9 Stable Cell Line-U2OS. Our stable cell line offers reliable and consistent Cas9 expression, ensuring efficient genome editing without the variability associated with transient transfection. By utilizing this advanced tool, researchers can streamline the generation of U2OS-CDDR cells with precise deletions in DNA repair genes, accelerating studies on DNA double-strand break repair mechanisms. Experience enhanced efficiency, reproducibility, and cost-effectiveness in your experiments with Creative Biogene's Cas9 Stable Cell Line-U2OS, your partner in cutting-edge genetic research.