Cas9 Stable Cell Line - CT26

Name: Cas9 Stable Cell Line - CT26
SKU: CSC-RO1150M

Cat.No. : CSC-RO1150M Host Cell: CT26

Size: >1x10^6 cells/vial Validation: T7 Endonuclease I assay

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Cell Line Information

Safety and Packaging

Cat. No.	CSC-RO1150M
Description	CT26-Cas9 cell line is engineered to stably express CRISPR Cas9 nuclease. This cell line also expresses hygromycin resistance gene. The Cas9 nuclease in this cell line has been functionally validated using T7 Endonuclease I assay. In combination with separately transfected sgRNAs, CT26-Cas9 cell line can be used to efficiently generate targeted genomic modifications including gene knockout, gene knockin, gene mutagenesis, gene tagging etc.
Introduction	Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology that contains two essential components: a guide RNA (gRNA) to match a target gene, and the Cas9 (CRISPR-associated protein 9) endonuclease which causes a double-stranded DNA break, allowing modifications to the genome via nonhomologous end joining (NHEJ) or homology-directed repair (HDR).
Target Gene	Cas9
Host Cell	CT26
Host Cell Species	Mus musculus (Mouse)
Product Type	Cas9 overexpression stable cell line
Applications	1) CRISPR genome editing, such as gene knockout (KO), gene knockin (KI), gene mutagenesis, gene tagging etc. 2) High-throughput sgRNA screening and validation
Quality Control	1) T7E1 assay 2) Mycoplasma detection
Size Form	One vial of frozen cells, typically >1x10^6 cells/vial
Shipping	Dry ice
Storage	Liquid nitrogen

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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