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Cas9 Stable Cell Line-SNU-475

Cat.No.
CSC-RO0036
Description
SNU-475-Cas9 is clonally derived from SNU-475 cell line which has been transfected with a Cas9/GFP/Hygro encoding vector, followed by stable cell selection. This cell line stably expresses CRISPR Cas9 nuclease, GFP and hygromycin resistance gene. The Cas9
Host Cell
SNU-475
Introduction
SNU-475-Cas9 is clonally derived from SNU-475 cell line which has been transfected with a Cas9/GFP/Hygro encoding vector, followed by stable cell selection. This cell line stably expresses CRISPR Cas9 nuclease, GFP and hygromycin resistance gene. The Cas9 nuclease in SNU-475-Cas9 cell line has been functionally validated using T7 Endonuclease I assay. In combination with separately transfected sgRNAs, SNU-475-Cas9 cell line can be used to efficiently generate targeted genomic modifications including gene knockout, gene knockin, gene mutagenesis, gene tagging etc. It is also an ideal cell line model for sgRNA screening and validation, either individually or in pools.
Source
SNU-475

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