All-in-One CRISPR/Cas9 KO Kit

Name: All-in-One CRISPR/Cas9 KO Kit
SKU: CC-01
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-01

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Gene Information

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Cat. No.	CC-01
Description	The CRISPR/Cas9 constructing and activity detecting mediated by All-in-One CRISPR/Cas9 KO Kit don’t need specific species cell line. Applying eukaryotic expression system, the All-in-One CRISPR/Cas9 KO Kit can be used for sgRNA activity detection and gene targeting in all mammals and relevant cell line. For non-mammals, the sgRNAs and targeting sequence can be cloned into the vectors of the kit for activity detection.
Gene Abbr	cas9
Report Gene	High Efficiency
Efficiency	5-20 min of digestion time is enough to detect any type of base mutations such as substitution, insertion and deletion.
Features	All-in-One CRISPR/Cas9 KO Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/Cas9. All-in-One CRISPR/Cas9 KO Kit is applicable to modify genes in almost all kinds of mammalian cells.
Applications	The All-in-One CRISPR/Cas9 KO Kit can be applied for activity detecting of many kinds of nucleases, such as CRISPR/Cas9, TALEN and ZFN and so on.
Manipulation	Linearized vector is provided and the DNA fragment encoding crRNA is less than 100 bp, which makes manipulation simpler. PCR product can be directly used for enzyme digestion without purification.
Sensitivity	The detecting sensitivity of the All-in-One CRISPR/Cas9 KO Kit way is higher than traditional mismatch-specific DNA endonuclease based detecting method.
Throughput	Hundreds of sgRNAs can be detected at one time.
Storage	Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Target Gene

Cas9

CC-01-All-in-One CRISPR/Cas9 KO Kit-Datasheet

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Which nuclease activities can this kit be used for?

A: The kit can be used to detect the activity of various nucleases, such as CRISPR/Cas9, TALEN and ZFN.

What is the mechanism of CRISPR/Cas-9 genome editing?

A: The mechanism of CRISPR/Cas-9 genome editing can be generally divided into three steps: recognition, cleavage, and repair. The designed sgRNA directs Cas-9 and recognizes the target sequence in the gene of interest through its 5?crRNA complementary base pair component.

How do I make a CRISPR KO cell line?

A: There are three main steps in the workflow to generate a CRISPR KO cell line:<br/>1. Design and make the CRISPR guide RNA<br/>2. Edit the cells by transfecting the CRISPR components (gRNA and Cas9)<br/>3. Analyze the editing results

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Customer Reviews

Simplify the Workflow

The kit is very friendly to new CRISPR users. Simplify the workflow and get results quickly.

United States

2022-12-17

Rapid & Simple

Very good. The digestion time of 5-20 minutes is enough to detect any type of base mutation, such as replacement, insertion and deletion.

United States

2022-12-18

Wide Range of Applications

The All-in-One CRISPR/Cas9 KO Kit is suitable for gene modification of almost all mammalian cells.

United States

2022-12-19

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All-in-One CRISPR/Cas9 KO Kit

Product Information

Gene Information

Documents

Q & A

Customer Reviews

Which nuclease activities can this kit be used for?

What is the mechanism of CRISPR/Cas-9 genome editing?

How do I make a CRISPR KO cell line?

Simplify the Workflow

Rapid & Simple

Wide Range of Applications

Related Products

Related Services