CRISPR/Cas9 KO Kit

Name: CRISPR/Cas9 KO Kit
SKU: CC-02
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-02

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Gene Information

Documents

Cat. No.	CC-02
Description	CRISPR/Cas9 KO Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/Cas9. Compared to the traditional TALEN and ZFN technologies for gene knockout, CRISPR/Cas9 KO Kit can be more easily manipulated and exhibits the highest knockout-efficiency. CRISPR/Cas9 KO Kit is applicable to modify genes in almost all kinds of mammalian cells.
Gene Abbr	cas9
Efficiency	Two functional components are combined into one vector which offers a higher transfection efficiency.
Features	The CRISPR/Cas9 KO Kit don’t need specific species cell line. Applying eukaryotic expression system, the CRISPR/Cas9 KO Kit can be used for sgRNA activity detection and gene targeting in all mammals and relevant cell line. For non-mammals, the sgRNAs and targeting sequence can be cloned into the vectors of the kit for activity detection.
Applications	The CRISPR/Cas9 KO Kit can be applied to modify genes in almost all kinds of mammalian cells.
Manipulation	Linearized vector is provided and the DNA fragment encoding crRNA is less than 100 bp, which makes manipulation simpler. PCR product can be directly used for enzyme digestion without purification.
Throughput	Hundreds of sgRNAs can be detected at one time.
Storage	Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Target Gene

Cas9

CC-02-CRISPR/Cas9 KO Kit-Datasheet

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Q & A

Customer Reviews

Customer Q&As

Is donor plasmid necessary for knocking out a target gene?

A: Without donor template DNA, the double-stranded break will be repaired by NHEJ, which tends to introduce unpredicted indels. You need to screen the deletions/insertions that cause frame shift of the target gene. Using donor DNA, you can get desired insertion/deletion/mutations. You can also add mammalian selection in donor DNA, which can greatly simplify your downstream screening procedures.

How many cell lines have been tested for CRISPR/Cas9 KO Kit?

A: CRISPR/Cas9 KO Kit has successfully tested in HeLa, HEK293T and MIA PaCa-2 (a human pancreatic carcinoma cell line).

If I do not have cells to survive after puromycin selection using CRISPR/Cas9 KO Kit, what is the reason?

A: There are two possible reasons: 1. This gene is an important cell survival gene, so it cannot tolerate the knockout of the constituent gene. Conditional elimination is required. 2. The transfection efficiency is too low. It is necessary to optimize the transfection or select different transfection methods, such as electroporation.

What are the advantages of our CRISPR/Cas9 KO Kit?

A: Although homology-directed recombination (HDR)-mediated gene knockout/knockin is well established, it may not be applicable to some cell types and organisms with low HDR efficiency. Our CRISPR/Cas9 KO Kit is specifically designed to provide universal solutions for the gene knockout needs of each cell type and organism.

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Customer Reviews

Worked Very Well

CRISPR/Cas9 KO Kit has simple operation and high knockout efficiency.

United States

2022-12-20

High Transfection Efficiency

Two functional components are combined into one vector which offers a higher transfection efficiency.

United States

2022-12-21

Wide Range of Applications

CRISPR/Cas9 KO Kit can be used for the detection of sgRNA activity and gene targeting of all mammalian and related cell lines.

United States

2022-12-17

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CRISPR/Cas9 KO Kit

Product Information

Gene Information

Documents

Q & A

Customer Reviews

Is donor plasmid necessary for knocking out a target gene?

How many cell lines have been tested for CRISPR/Cas9 KO Kit?

If I do not have cells to survive after puromycin selection using CRISPR/Cas9 KO Kit, what is the reason?

What are the advantages of our CRISPR/Cas9 KO Kit?

Worked Very Well

High Transfection Efficiency

Wide Range of Applications

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