Cas9 Stable Cell Line - 1321N1

Name: Cas9 Stable Cell Line - 1321N1
SKU: CSC-RO0224
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0224 Host Cell : 1321N1

Size : >1x10⁶ cells/vial Validation : T7 Endonuclease I assay

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0224
Description	1321N1-Cas9 cell line is engineered to stably overexpress Cas9 nuclease. The Cas9 nuclease in 1321N1-Cas9 cell line has been functionally validated using T7 Endonuclease I assay. In combination with separately transfected sgRNAs, 1321N1-Cas9 cell line can be used to efficiently generate targeted genomic modifications including gene knockout, gene knockin, gene mutagenesis, gene tagging etc. It is also an ideal cell line model for sgRNA screening and validation, either individually or in pools.
Introduction	Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology that contains two essential components: a guide RNA (gRNA) to match a target gene, and the Cas9 (CRISPR-associated protein 9) endonuclease which causes a double-stranded DNA break, allowing modifications to the genome via nonhomologous end joining (NHEJ) or homology-directed repair (HDR).
Product Type	Cas9 overexpression stable cell line
Target Gene	Cas9
Host Cell	1321N1
Host Cell Species	Homo sapiens (Human)
Applications	1) CRISPR genome editing, such as gene knockout (KO), gene knockin (KI), gene mutagenesis, gene tagging etc. 2) High-throughput sgRNA screening and validation
Size	One vial of frozen cells, typically >1x10⁶ cells/vial
Validation	T7 Endonuclease I assay
Quality Control	1) T7E1 assay 2) Mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

Cas9

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