Cas9 Stable Cell Line - IPEC-J2

The IPEC-J2 cell line is a non-transformed intestinal epithelial cell line derived from the jejunum of newborn piglets. It is an important in vitro model for studying porcine intestinal physiology, barrier function, and host-pathogen interactions, and is applied in porcine biology and comparative biology. The Cas9 Stable Cell Line - IPEC-J2 is a genetically engineered variant in which CRISPR-associated protein 9 (Cas9) has been stably integrated into the genome of IPEC-J2 cells. This modification allows for stable and efficient genome editing without transient transfection of the Cas9 component. Stable expression of Cas9 reduces experimental variability, simplifies gene knockout or knock-in studies, and makes it ideal for high-throughput screening. These cells retain key characteristics of the parent cell line, including epithelial morphology, polarization, and barrier integrity, while providing a powerful platform for precise gene manipulation.

The Cas9 Stable Cell Line - IPEC-J2 is widely used to study intestinal diseases such as inflammatory bowel disease (IBD), necrotizing enterocolitis (NEC), and viral/bacterial infections (e.g., rotavirus or Salmonella infections). Researchers have used this cell line to construct specific gene knockout models involving immune responses (such as TLRs or cytokines), nutrient transporters, or tight junction proteins, thereby elucidating their roles in intestinal barrier dysfunction. Furthermore, this cell line can aid in drug development by screening compounds that modulate epithelial repair or pathogen resistance. In toxicology, it helps assess the effects of environmental toxins, feed additives, or nanomaterials on gut health. The cell line also supports research on host-microbiome interactions, allowing for targeted editing to explore how gut microbes influence epithelial gene expression.