Cas9 Stable Cell Line - HT-22

The HT-22 cell line is an immortalized mouse hippocampal neuronal cell line originally derived from primary mouse neurons. Due to its stability and relevance in studying neuronal function, oxidative stress responses, and neurodegenerative pathways, these cells are widely used in neuroscience research. The Cas9 Stable Cell Line - HT-22 is a genetically engineered variant in which CRISPR-associated protein 9 (Cas9) is stably integrated into the HT-22 genome. This modification ensures stable, high-level expression of the Cas9 endonuclease, eliminating the need for transient transfection in gene-editing experiments. This engineered cell line provides a reliable platform for targeted gene manipulation, accelerating research in neurobiology and molecular genetics.

The Cas9 Stable Cell Line - HT-22 has broad application prospects in multiple research fields. In functional genomics, it enables efficient gene knockout, knock-in, or activation studies using CRISPR-Cas9 technology, allowing researchers to explore gene function in neuronal development, apoptosis, and synaptic plasticity. In modeling neurodegenerative diseases, this cell line can construct homologous models of Alzheimer''s disease, Parkinson''s disease, or Huntington''s disease by targeting genes such as APP, SNCA, or HTT. In drug development, it supports high-throughput screening of neuroprotective compounds targeting oxidative stress or tau protein pathways. Furthermore, stable Cas9 expression simplifies the CRISPR library screening process, facilitating the identification of novel therapeutic targets in neuroinflammation or autophagy regulation.