Cas9 Stable Cell Line - 293T

The Cas9-stable 293T cell line is a genetically engineered derivative of the human embryonic kidney cell line HEK 293T; the original HEK 293T line was established through the adenovirus-mediated transformation of primary kidney cells. This modified cell line is capable of stably integrating the CRISPR-associated protein 9 (Cas9) nuclease, thereby enabling continuous genomic editing capabilities without the need for repeated transfections. As a derivative of HEK 293 cells—which express the SV40 Large T antigen—its parental 293T cell line is characterized by exceptionally high transfection efficiency, robust protein expression capabilities, and highly efficient viral packaging capacity. The Cas9-stable 293T cell line is renowned for its superior transfection efficiency, remarkably short doubling time, and excellent compatibility with a wide variety of delivery methods. It not only retains the epithelial morphological characteristics of its parental cells but also consistently maintains stable and efficient genomic editing capabilities throughout successive passages.

In the field of functional genomics, the Cas9-stable 293T cell line facilitates genome-wide CRISPR knockout screens, enabling the identification of genes that play pivotal roles in viral replication, signal transduction pathways (such as NF-κB), and DNA damage response mechanisms. Researchers can leverage its exceptionally high transfection efficiency to rapidly generate isogenic cell models—either by knocking in disease-associated mutations (e.g., cancer-driving mutations) or by correcting genetic defects—thereby facilitating in-depth investigations into the pathological mechanisms underlying various conditions, such as hereditary metabolic disorders. In virology research, this cell line significantly advances the elucidation of host-pathogen interaction mechanisms through the targeted knockout of viral receptors or host restriction factors. In biopharmaceutical manufacturing, the cell line streamlines the generation of stable clonal lines for recombinant protein expression by enabling the precise editing of genes involved in glycosylation modifications or protein secretion pathways.