Cytology

The Essential Principles of Constructing Knock-In Cell Lines and Key Details to Remember

Knock-in (KI) cell lines are crucial tools in biomedical research, predominantly used to model diseases, perform gene function analyses, and develop therapeutic strategies. Their construction involves integrating a specific allele or genetic alteration into a particular locus on the cell's chromosome.

The Key to Moving Gene Therapy from the Clinic to Commercialization Scaling Up the AAV Production Process

As gene therapy is experiencing a second wave of interest, recombinant adeno-associated virus (rAAV) has rapidly emerged as one of the most attractive viral transfer tools, thanks to its favorable safety profile and long-term transgene expression. The recent approval of rAAV molecules for the treatment of congenital amaurosis and spinal muscular atrophy has been paving the road for the development of several rAAV-based therapeutics targeting a continuously increasing range of indications.

Rapid Development and Scale-Up of Biosimilars: A Case Study of Cell Line and Process Development

Increasing availability of genomic and transcriptomic resources for industrially important cell lines coupled with advances in genome editing technology have opened new avenues for cell line development. These developments are poised to help biosimilar manufacturing, which requires targeting pre-defined product quality attributes, e.g., glycoform, to match the innovator's range.

High-Performance CHO-Stabilized Cell Lines for Biopharmaceutical Development

Biological therapeutics and diagnostics (theranostics) are rapidly growing products in the pharmaceutical market. They include various monoclonal antibodies (mAbs), vaccines, hormones, and other proteins, all of which have wide range of applications. Biotherapeutic proteins represent a mainstay of treatment for a multitude of conditions, for example, autoimmune disorders, hematologic disorders, hormonal dysregulation, cancers, infectious diseases and genetic disorders.

Immune Receptor Stable Cell Lines: A Useful Tool in Aiding Anti-Tumor Novel Drugs Development

Immune checkpoints are the crucial regulators of immune system and play essential roles in maintaining self-tolerance, preventing autoimmune responses, and minimizing tissue damage by regulating the duration and intensity of the immune response.

Application Prospects of Reporter Cell Lines

The reporter cell lines have shown great potential for cell tracing, drug development and gene function studies, etc. Creative Biogene offers reporter cell lines with high sensitivity and accurate localization, which have become a powerful tool of choice for researchers.

Application of Kinase Stable Cell Lines Detection of Secondary EGFR Mutations in Afatinib-Resistant BaF3 Cells

Kinases serve as key regulatory molecules for most cellular pathways, which are often associated with diseases: as causative agents for diseases, especially many cancers, or as targets for drug development.

Application of HEK293 Cell Line in the Production of Recombinant Proteins and Viral Vectors

The HEK293 cell line was established by transforming human embryonic kidney cells with sheared adenovirus type 5 DNA. Since then, many subtypes and derivatives have been established, with HEK293, HEK293-T, and HEK293-F frequently used in the production of biopharmaceuticals.

The Role of CAR-NK Cells in Cancer Cellular Immunotherapy

This article describes the unique advantages of CAR-NK cells, their production and application in cancer cell immunotherapy.

Research of Genome-wide CRISPR Screening in Primary Human T Cells among Key Regulators of Immune Function

Cytotoxic T cells play a central role in immune-mediated control of cancer, infectious diseases, and autoimmunity. Genome-scale CRISPR screens in primary human T cells would enable comprehensive target discovery studies that could be rapidly translated into new immunotherapies with small molecules, biologics, and gene-engineered adoptive cell therapies.

* For research use only. Not intended for any clinical use.

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