Cat.No. : CSC-RO0450 Host Cell: MC38
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0450 |
| Description | This cell line is derived from MC38 and is engineered to stably overexpress Human EGFR. |
| Target Gene | EGFR |
| Gene Species | Homo sapiens (Human) |
| Host Cell | MC38 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Enhancing antibody-dependent cellular cytotoxicity (ADCC) is a promising strategy to improve the efficacy of tumor-targeted therapies, especially monoclonal antibodies. ADCC involves the activation of immune cells, particularly NK cells, to destroy tumor cells. The researchers focused on improving ADCC through the use of a recombinant chimeric protein, Sushi-IL15-Apo, which combines the sushi domain of IL-15Rα, IL-15, and apolipoprotein A-I (Apo). This protein promotes NK cell proliferation, survival, and cytotoxicity, thus increasing the effectiveness of tumor-targeted antibodies. They also demonstrated that SR-B1, the receptor for ApoA-I, is expressed on tumor cells, enabling the chimeric protein to interact with both tumor cells and NK cells, enhancing the ADCC response. The researchers used the MC38-EGFR cell line to test the effect of Sushi-IL15-Apo in combination with anti-EGFR antibodies.
Figure 1. The researchers assessed the anti-tumor effect of Sushi-IL15-Apo in a subcutaneous syngeneic model using MC38-EGFR colon cancer cells. Tumor volume was monitored in mice treated with anti-EGFR antibodies and Sushi-IL15-Apo plasmid, showing enhanced tumor suppression and improved survival, with statistical significance indicated by p-values. (Ochoa MC, et al., 2017)
Creative Biogene's Human EGFR Stable Cell Line - MC38 is well-suited for similar ADCC-focused studies, providing a reliable platform for testing therapeutic strategies targeting EGFR in cancer research.
A: Researchers can utilize the Human EGFR Stable Cell Line - MC38 to investigate various cellular processes, including cell proliferation, migration, and survival signaling. By analyzing these processes in a system that stably expresses human EGFR, scientists can gain insights into the molecular mechanisms by which EGFR contributes to colorectal cancer progression. This includes the study of downstream signaling pathways such as the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways, which are often dysregulated in cancer.
A: The Human EGFR Stable Cell Line - MC38 can be used to create in vitro and in vivo models to study the tumor microenvironment. By comparing the interactions between MC38 cells with and without EGFR overexpression and immune cells, researchers can evaluate how EGFR levels influence immune cell recruitment, activation, and function. This can provide valuable information on the immunomodulatory effects of EGFR and inform the development of immunotherapeutic strategies for colorectal cancer.
A: The Human EGFR Stable Cell Line - MC38 can be instrumental in the development of personalized cancer treatments by serving as a platform for testing the effects of various EGFR-targeted therapies. By introducing patient-derived EGFR mutations into the MC38 cells, researchers can assess the efficacy of specific drugs or drug combinations on a case-by-case basis. This can help identify the most effective treatment strategies for individual patients based on their unique genetic profile.
A: The Human EGFR Stable Cell Line - MC38 can be employed to study resistance mechanisms by subjecting the cells to EGFR inhibitors over an extended period, allowing for the selection of resistant cell variants. These resistant cells can then be analyzed to identify molecular changes, such as secondary mutations in EGFR or upregulation of alternative signaling pathways, that confer resistance to therapy. Understanding these mechanisms can guide the development of combination therapies or new treatment strategies to overcome resistance.
A: With the Human EGFR Stable Cell Line - MC38, researchers can develop various high-throughput screening assays to identify novel EGFR modulators. These assays may include cell-based assays that measure changes in cell viability, proliferation, or signaling readouts in response to compound treatments. Additionally, reporter gene assays that detect the activation of EGFR-responsive elements can be utilized. The identified compounds can then be further characterized for their potential as therapeutic agents for the treatment of colorectal and other EGFR-driven cancers.
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The Human FGFR4-N535K Stable Cell Line - BaF3 accurately represents the N535K mutation in FGFR4, providing a reliable model for studying specific mutation effects. This accurate modeling is essential for understanding how this particular mutation influences cell behavior and signaling pathways.
This cell line ensures stable expression of the FGFR4-N535K mutation, allowing for consistent experimental conditions. The Human FGFR4-N535K Stable Cell Line - BaF3's stability is crucial for obtaining reproducible results across multiple experiments and research studies.
Benefiting from the robust nature of the BaF3 cell background, the Human FGFR4-N535K Stable Cell Line - BaF3 supports effective growth and sustainability in various experimental setups. This robust performance is advantageous for conducting extensive and demanding research protocols.
The cell line is well-prepared for developing and conducting biochemical and cellular assays that require the mutated FGFR4. The Human FGFR4-N535K Stable Cell Line - BaF3 is particularly useful for assays that investigate kinase activity, receptor binding, and signal transduction.
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