Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2307 Host Cell : HEK293
| Cat. No. | CLOE-2307 |
| Introduction | This cell lysate over-expresses mouse Egfr (Q01279) (Met 1-Ser 647) fused with C-terminal his tag. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | Egfr |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Egfr epidermal growth factor receptor [ Mus musculus ] |
| Gene Symbol | Egfr |
| Synonyms | Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik |
| Gene Description | epidermal growth factor receptor |
| Gene ID | 13649 |
| Uni Prot ID | Q9WVF5 |
| m RNA Refseq | NM_007912.4 |
| Protein Refseq | NP_031938.1 |
| Chromosome Location | 11 A1-A4; 11 9.0 cM |
| Function | ATP binding; actin filament binding; enzyme binding; epidermal growth factor binding; epidermal growth factor-activated receptor activity; epidermal growth factor-activated receptor activity; glycoprotein binding; identical protein binding; integrin binding; kinase activity; nucleotide binding; protein binding; protein heterodimerization activity; protein kinase activity; protein kinase binding; protein phosphatase binding; protein tyrosine kinase activity; receptor binding; receptor signaling protein tyrosine kinase activity; signal transducer activity; transferase activity; transferase activity, transferring phosphorus-containing groups; transmembrane receptor protein tyrosine kinase activity; transmembrane signaling receptor activity; |
| Pathway | Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Bladder cancer, organism-specific biosystem; |
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