Cat.No. : CSC-RO0314 Host Cell: Ba/F3
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0314 |
| Description | This cell line is engineered to stably overexpress human EGFR bearing P772-H773_ins_NP mutation. |
| Target Gene | EGFR |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Ba/F3 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Growth Conditions | 37 °C, 5% CO2 |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Biosafety Level | 2 |
| Thawing & Subculturing Instructions | 1. Thaw cells by gently swirling in a 37°C water bath. To limit contamination, do not submerge the O-ring and cap. 2. When cells are ~70% thawed (~1 min), transfer the vial into a biosafety cabinet, and wipe the surface with 70% ethanol. Allow tube to dry completely. 3. Transfer the cells gently into a 15 mL conical tube containing 10 mL of pre-warmed culture medium (without antibiotic selection marker). Centrifuge cells at ~125 x g for 5~7 min. 4. Remove supernatant without disturbing the pellet, and resuspend cells in 1 mL culture medium (without antibiotic selection marker). Transfer cells to a 6-well plate containing ~2 mL pre-warmed growth medium (without antibiotic selection marker) or a T25 flask containing 5 mL pre-warmed culture medium (without antibiotic selection marker). 5. Incubate the culture at 37°C with 5% CO2. 6. Subculture: split saturated culture 1:4 ~ 1:6 every 3 days; seed out at about 1~3 x 10^5 cells/mL. |
| Growth Properties | Suspension, round |
| Freeze Medium | Frozen with 70% medium, 20% FBS, 10% DMSO |
| Freezing Instructions | Cells are recommended to generate additional frozen stocks at early passages. Frozen stocks should be preserved in a designated cryopreservation medium or in 70% RPMI 1640 + 20% FBS + 10% DMSO (without antibiotic selection marker). 1. Prepare the freezing medium (70% RPMI 1640 + 20% FBS + 10% DMSO, without antibiotic selection marker) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250 x g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3 x10^6 cells/ml in chilled freezing medium. 6. Aliquot 1 ml of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
EGFR exon 20 insertions are a common mutation in non-small cell lung cancer (NSCLC) that are often associated with poor prognosis and resistance to first- and second-generation EGFR tyrosine kinase inhibitors (TKIs). These mutations can vary in location within the exon, affecting the tumor's response to targeted therapies. The researchers in this study explored the efficacy of poziotinib, a third-generation TKI, in treating EGFR exon 20 mutant NSCLC. Their findings showed that the insertion site significantly influences the drug's activity, with near-loop insertions showing higher sensitivity to poziotinib than far-loop insertions. In addition, the study identified various mechanisms of acquired resistance, including EGFR T790M mutations and epithelial-to-mesenchymal transition (EMT).
Figure 1. The researchers identified potential acquired resistance mechanisms to poziotinib in 14 out of 23 NSCLC patients by analyzing matched pre-treatment and progression samples. (Elamin YY, et al., 2022)
For similar research, Creative Biogene's Human EGFR-P772-H773_ins_NP Stable Cell Line - Ba/F3 can be utilized to model EGFR exon 20 insertions. This cell line provides a reliable platform for studying the effects of EGFR mutations and evaluating drug responses, making it an ideal tool for testing potential therapeutics like poziotinib.
A: Our human EGFR-P772-H773_ins_NP stable cell line has undergone long-term stability testing and maintains stable expression levels over multiple passages. We regularly validate cell lines and record cell line stability data. Customers can request this data from us to understand the stability performance of our products.
A: Our human EGFR-P772-H773_ins_NP stable cell line is fully functional and has been widely used in anticancer drug sensitivity studies. We provide detailed functional verification data, including cell proliferation experiments, cell apoptosis experiments, etc. Customers can refer to these data to evaluate the performance of our products.
A: Our human EGFR-P772-H773_ins_NP stable cell line has been validated in personalized medicine research. We provide relevant preclinical research data support to prove the application potential of our products in these fields.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I am very impressed with this Human EGFR-P772-H773_ins_NP stable cell line. First of all, I have to say that the professionalism of the vendors is admirable. Their service attitude was very cordial and they gave detailed answers to my questions, which gave me a clearer understanding of the product. Secondly, the stability of the cell lines is also amazing. During the culture process, I observed very consistent cell growth and very stable gene expression. This has greatly helped my research work, allowing me to focus more on the analysis and interpretation of experimental results.
For me, this Human EGFR-P772-H773_ins_NP stable cell line is a blessing. I've had a lot of problems with other cell lines in the past, but this was completely different. First, I would like to thank the supplier for their quality assurance. Their products are not only reliable in quality but also very stable, allowing me to experiment with confidence. Secondly, these cells showed astonishing growth rates during culture. I observed that cells grew in a very consistent manner and gene expression was very stable, which provided strong support for my research.
Throughout my use of the Human EGFR-P772-H773_ins_NP stable cell line, I have received top-notch service and product quality. First, I would like to commend the supplier for their professionalism. They knew the product well and were very patient when answering my questions, which made me feel their professionalism and responsible attitude. Secondly, I was impressed by the stability of the cell line. During the culture process, I observed that the cells grew very healthily and the gene expression was very stable, which provided a good foundation for my research work.
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