Cat.No. : CSC-RO0141 Host Cell: Ba/F3
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0141 |
| Description | Ba/F3-EGFR-T790M cell line is a stably transfected cell line which expresses human epidermal growth factor receptor (EGFR) with T790M mutation. |
| Target Gene | EGFR |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Ba/F3 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Growth Conditions | 37 °C, 5% CO2 |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Biosafety Level | 2 |
| Thawing & Subculturing Instructions | 1. Thaw cells by gently swirling in a 37°C water bath. To limit contamination, do not submerge the O-ring and cap. 2. When cells are ~70% thawed (~1 min), transfer the vial into a biosafety cabinet, and wipe the surface with 70% ethanol. Allow tube to dry completely. 3. Transfer the cells gently into a 15 mL conical tube containing 10 mL of pre-warmed culture medium (without antibiotic selection marker). Centrifuge cells at ~125 x g for 5~7 min. 4. Remove supernatant without disturbing the pellet, and resuspend cells in 1 mL culture medium (without antibiotic selection marker). Transfer cells to a 6-well plate containing ~2 mL pre-warmed growth medium (without antibiotic selection marker) or a T25 flask containing 5 mL pre-warmed culture medium (without antibiotic selection marker). 5. Incubate the culture at 37°C with 5% CO2. 6. Subculture: split saturated culture 1:4 ~ 1:6 every 3 days; seed out at about 1~3 x 10^5 cells/mL. |
| Growth Properties | Suspension, round |
| Freeze Medium | Frozen with 70% medium, 20% FBS, 10% DMSO |
| Freezing Instructions | Cells are recommended to generate additional frozen stocks at early passages. Frozen stocks should be preserved in a designated cryopreservation medium or in 70% RPMI 1640 + 20% FBS + 10% DMSO (without antibiotic selection marker). 1. Prepare the freezing medium (70% RPMI 1640 + 20% FBS + 10% DMSO, without antibiotic selection marker) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250 x g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3 x10^6 cells/ml in chilled freezing medium. 6. Aliquot 1 ml of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
About 60% of lung cancer patients with sensitizing EGFR mutations and disease progression following first- and second-generation EGFR TKI therapy had the EGFR T790M mutation, which contributes to acquired resistance. To study T790M-positive cell treatment sensitivity, the researchers used patient-derived cell lines such the EGFR-KDDT790M Ba/F3 models. They identified the T790M mutation in a patient whose tumor developed this mutation as a mechanism to resist erlotinib treatment. Their findings suggest that the EGFR C797S mutation may also contribute to resistance against osimertinib, emphasizing the complexity of resistance mechanisms.
Figure 1. The researchers established various T790M-positive EGFR-KDD Ba/F3 models, including EGFR-KDDD1T, EGFR-KDDD2T, and EGFR-KDDBDT, to characterize the mutation's properties. They conducted cell viability and immunoblot assays, aiming to assess the sensitivity of these cell lines to different EGFR TKIs, revealing significant resistance to first- and second-generation inhibitors. (Lee C, et al., 2021)
The Human EGFR-T790M Stable Cell Line-Ba/F3 from Creative Biogene lets researchers study the mutation's effects in a controlled setting. This cell line is valuable for testing T790M-positive cells' EGFR TKI sensitivity, especially third-generation inhibitors.
A: Human EGFR-T790M Stable Cell Line-Ba/F3 has undergone strict gene editing and screening to ensure the high stability of the EGFR-T790M mutation. The cell line is cultured and amplified over multiple generations to ensure mutations do not drift. Experimental data showed that the expression levels and functions of the mutant genes remained consistent during long-term culture (more than 30 generations). This high stability makes it a reliable tool for studying cancer mechanisms and drug screening related to EGFR-T790M mutations.
A: This cell line specifically mimics the drug resistance caused by the EGFR-T790M mutation, especially showing obvious resistance in experiments against EGFR inhibitors such as erlotinib and gefitinib. This resistance is highly correlated with clinically observed drug resistance in patients, making this cell line an ideal model for studying resistance mechanisms and screening novel drugs against EGFR mutations. The results showed that the cell line showed a consistent drug resistance response when treated with different concentrations of inhibitors.
A: Yes, detailed cell line certification and identification reports are provided. Customers can request STR (short tandem repeat) analysis results, gene mutation detection reports, cell morphology photos and gene expression analysis. These reports are certified by authoritative laboratories to ensure the identity and quality of the cell lines. Customers can use these reports to verify the authenticity and stability of cell lines and ensure the reliability and reproducibility of experimental results.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I recently used the Human EGFR-T790M Stable Cell Line-Ba/F3 for experiments, and the overall experience was excellent. This cell line stably expresses the T790M mutation of the human epidermal growth factor receptor (EGFR) and provides an important tool for studying EGFR-TKI resistance.
Human EGFR-T790M Stable Cell Line-Ba/F3 is a very efficient research model, especially suitable for studying the mechanism of EGFR-TKI resistance. This cell line stably expresses the T790M mutation of EGFR, simulating a common clinical resistance mechanism. Using this cell line for experiments, I obtained stable and reliable results. The service provided by the merchant was also very timely and professional, and they gave me full support and guidance.
The stability and consistency of the cell lines makes my research run more smoothly. The service quality provided by the boss is also very high. They responded quickly and provided professional technical support, which greatly improved my experimental efficiency.
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