Cat.No. : CSC-RO0128 Host Cell: Ba/F3
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0128 |
| Description | Ba/F3-EGFR-D770_N771insSVD cell line is a stably transfected cell line which expresses human epidermal growth factor receptor (EGFR) with D770_N771insSVD mutation. |
| Target Gene | EGFR |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Ba/F3 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Growth Conditions | 37 °C, 5% CO2 |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Biosafety Level | 2 |
| Thawing & Subculturing Instructions | 1. Thaw cells by gently swirling in a 37°C water bath. To limit contamination, do not submerge the O-ring and cap. 2. When cells are ~70% thawed (~1 min), transfer the vial into a biosafety cabinet, and wipe the surface with 70% ethanol. Allow tube to dry completely. 3. Transfer the cells gently into a 15 mL conical tube containing 10 mL of pre-warmed culture medium (without antibiotic selection marker). Centrifuge cells at ~125 x g for 5~7 min. 4. Remove supernatant without disturbing the pellet, and resuspend cells in 1 mL culture medium (without antibiotic selection marker). Transfer cells to a 6-well plate containing ~2 mL pre-warmed growth medium (without antibiotic selection marker) or a T25 flask containing 5 mL pre-warmed culture medium (without antibiotic selection marker). 5. Incubate the culture at 37°C with 5% CO2. 6. Subculture: split saturated culture 1:4 ~ 1:6 every 3 days; seed out at about 1~3 x 10^5 cells/mL. |
| Growth Properties | Suspension, round |
| Freeze Medium | Frozen with 70% medium, 20% FBS, 10% DMSO |
| Freezing Instructions | Cells are recommended to generate additional frozen stocks at early passages. Frozen stocks should be preserved in a designated cryopreservation medium or in 70% RPMI 1640 + 20% FBS + 10% DMSO (without antibiotic selection marker). 1. Prepare the freezing medium (70% RPMI 1640 + 20% FBS + 10% DMSO, without antibiotic selection marker) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250 x g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3 x10^6 cells/ml in chilled freezing medium. 6. Aliquot 1 ml of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Non-small-cell lung cancer (NSCLC) is a complex and challenging disease, especially when driven by uncommon epidermal growth factor receptor (EGFR) mutations, which account for 10%–20% of EGFR mutations in NSCLC. Patients with these mutations, including the EGFR-D770_N771insSVD variant, often exhibit poor clinical outcomes and limited responses to standard EGFR tyrosine kinase inhibitors (TKIs) such as afatinib and osimertinib. Researchers have highlighted the need for novel therapies, focusing on the development of next-generation EGFR-TKIs like aumolertinib. Recent studies demonstrate aumolertinib's promising in vitro efficacy, as it effectively inhibits cell viability in Ba/F3 models harboring various uncommon EGFR mutations and significantly curbs tumor growth in vivo in mouse models. These findings suggest its potential as a new therapeutic candidate for EGFR-mutated NSCLC.
Figure 1. The researchers used Ba/F3 cells with EGFR mutations, including EGFR-D770_N771insSVD, to assess aumolertinib's inhibitory activity. Their goal was to explore selective anticancer effects against non-small-cell lung cancer (NSCLC) driven by these mutations. (Shi C, et al., 2023)
Creative Biogene offers the Human EGFR-D770_N771insSVD Stable Cell Line-Ba/F3, a crucial tool for similar research into EGFR-TKI sensitivity and resistance mechanisms. This stable cell line enables researchers to explore drug responses and resistance pathways, advancing the development of therapies tailored to uncommon EGFR mutations.
A: The Human p95HER2 Stable Cell Line-T47D was established to study the role of HER2 in the development of breast cancer. In breast cancer research, this cell line is particularly important because it stably expresses the p95HER2 protein, an active fragment of the HER2 receptor, closely associated with the aggressiveness and treatment resistance of breast cancer. By using this cell line, researchers can gain deeper insights into the specific role of p95HER2 in breast cancer progression, thereby providing a crucial basis for the development of targeted therapies.
A: The construction of the Human p95HER2 Stable Cell Line-T47D involved stably integrating the p95HER2 gene sequence into the genome of the T47D breast cancer cell line. Technical challenges in this process included selecting an appropriate vector to ensure stable gene expression and screening for cell clones that truly stably express the target protein. These challenges were successfully overcome using specific antibiotic selection markers and immunoblotting techniques to validate expression levels, ensuring the cell line's high specificity and functionality.
A: The Human p95HER2 Stable Cell Line-T47D exhibits its unique value in the research fields of breast cancer biomarker studies, screening and evaluation of new therapeutic drugs, and studying the role of HER2-related signaling pathways in cancer development. Especially in developing and testing therapeutic strategies for tumors expressing p95HER2, this cell line provides a valuable model for understanding the mechanisms of drug action and optimizing treatment protocols.
A: Compared to other breast cancer cell lines, the main advantage of the Human p95HER2 Stable Cell Line-T47D lies in its stable expression of p95HER2, a protein closely associated with the aggressiveness and treatment resistance of breast cancer. This characteristic makes the cell line an ideal choice for studying the role of p95HER2 in breast cancer development and provides an accurate model for evaluating therapeutic strategies targeting p95HER2. Therefore, it offers more targeted and practical information in simulating disease progression and treatment responses than other cell lines.
A: The Human p95HER2 Stable Cell Line-T47D can be used to study the metastasis mechanism of breast cancer, especially how p95HER2 affects the invasiveness and migratory capacity of cancer cells. By analyzing the behavior of this cell line under different conditions (such as different extracellular matrix components), researchers can reveal the role of p95HER2 in promoting breast cancer cell migration and invasion, providing targets to block cancer metastasis.
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The Human EGFR-D770_N771insSVD Stable Cell Line-Ba/F3 offers stable expression suitable for long-term research.
This product maintains the biological activity of EGFR-D770_N771insSVD, facilitating studies on its biological functions.
With this stable cell line, you can initiate experiments quickly, saving time and resources.
Suitable for various experimental designs and research directions, providing flexibility for researchers in different fields.
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