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Cat.No. : CSC-RO0132 Host Cell: Ba/F3
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0132 |
| Description | Ba/F3-EGFR-DEL19/T790M/C797S cell line is a stably transfected cell line which expresses human epidermal growth factor receptor (EGFR) with DEL19, T790M and C797S mutations. |
| Target Gene | EGFR |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Ba/F3 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Growth Conditions | 37 °C, 5% CO2 |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Biosafety Level | 2 |
| Thawing & Subculturing Instructions | 1. Thaw cells by gently swirling in a 37°C water bath. To limit contamination, do not submerge the O-ring and cap. 2. When cells are ~70% thawed (~1 min), transfer the vial into a biosafety cabinet, and wipe the surface with 70% ethanol. Allow tube to dry completely. 3. Transfer the cells gently into a 15 mL conical tube containing 10 mL of pre-warmed culture medium (without antibiotic selection marker). Centrifuge cells at ~125 x g for 5~7 min. 4. Remove supernatant without disturbing the pellet, and resuspend cells in 1 mL culture medium (without antibiotic selection marker). Transfer cells to a 6-well plate containing ~2 mL pre-warmed growth medium (without antibiotic selection marker) or a T25 flask containing 5 mL pre-warmed culture medium (without antibiotic selection marker). 5. Incubate the culture at 37°C with 5% CO2. 6. Subculture: split saturated culture 1:4 ~ 1:6 every 3 days; seed out at about 1~3 x 10^5 cells/mL. |
| Growth Properties | Suspension, round |
| Freeze Medium | Frozen with 70% medium, 20% FBS, 10% DMSO |
| Freezing Instructions | Cells are recommended to generate additional frozen stocks at early passages. Frozen stocks should be preserved in a designated cryopreservation medium or in 70% RPMI 1640 + 20% FBS + 10% DMSO (without antibiotic selection marker). 1. Prepare the freezing medium (70% RPMI 1640 + 20% FBS + 10% DMSO, without antibiotic selection marker) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250 x g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3 x10^6 cells/ml in chilled freezing medium. 6. Aliquot 1 ml of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Non-small cell lung cancer (NSCLC) is often driven by mutations in the epidermal growth factor receptor (EGFR) gene, with triple mutations such as Del19/T790M/C797S being particularly associated with resistance to standard therapies like osimertinib. Researchers have been investigating novel strategies to overcome this resistance, including the combination of new drugs. In a recent study, WX-0593, a potent anaplastic lymphoma kinase (ALK) inhibitor, demonstrated a synergistic effect when used alongside EGFR monoclonal antibodies, significantly inhibiting the growth of EGFR triple-mutant cell lines, particularly in Ba/F3 cells harboring the Del19/T790M/C797S mutation. This work highlights the importance of exploring combination therapies in addressing the challenges posed by resistant NSCLC.
Figure 1. The researchers examined the impact of WX-0593 combined with Vectibix on the proliferation of four EGFR triple-mutant cell lines, revealing significant synergy in Ba/F3 cells. (Zheng Q, et al., 2022)
Creative Biogene's Human EGFR-Del19/T790M/C797S Stable Cell Line-Ba/F3 serves as a powerful tool for researchers investigating EGFR resistance mechanisms and testing therapeutic efficacy. These stable cell lines are ideal for in vitro screening and targeted therapy studies, aiding the development of innovative treatment strategies for EGFR-mutant NSCLC.
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For us graduate students, using the Human EGFR-DEL19/T790M/C797S Stable Cell Line Ba/F3 product can greatly simplify the experimental steps, reduce experimental time, and thus have more energy to invest in data analysis and graduation thesis writing. It's really great and recommended.
French
06/20/2021
Due to the high efficiency and stability of the product, the materials and resources required for the experiment have been effectively saved, and the experimental cost has been reduced.
Canada
12/19/2023
By using this product, we can quickly initiate experiments and obtain reliable results, which plays a crucial role in promoting the research progress of DEL19/T790M.
French
11/13/2020
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