Cat.No. : CSC-RO0239 Host Cell: Ba/F3
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0239 |
| Description | This cell line stably expresses human EGFR gene bearing exon19 E746_A750 deletion, L858R and T790M triple mutations |
| Target Gene | EGFR |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Ba/F3 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Growth Conditions | 37 °C, 5% CO2 |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Biosafety Level | 2 |
| Thawing & Subculturing Instructions | 1. Thaw cells by gently swirling in a 37°C water bath. To limit contamination, do not submerge the O-ring and cap. 2. When cells are ~70% thawed (~1 min), transfer the vial into a biosafety cabinet, and wipe the surface with 70% ethanol. Allow tube to dry completely. 3. Transfer the cells gently into a 15 mL conical tube containing 10 mL of pre-warmed culture medium (without antibiotic selection marker). Centrifuge cells at ~125 x g for 5~7 min. 4. Remove supernatant without disturbing the pellet, and resuspend cells in 1 mL culture medium (without antibiotic selection marker). Transfer cells to a 6-well plate containing ~2 mL pre-warmed growth medium (without antibiotic selection marker) or a T25 flask containing 5 mL pre-warmed culture medium (without antibiotic selection marker). 5. Incubate the culture at 37°C with 5% CO2. 6. Subculture: split saturated culture 1:4 ~ 1:6 every 3 days; seed out at about 1~3 x 10^5 cells/mL. |
| Growth Properties | Suspension, round |
| Freeze Medium | Frozen with 70% medium, 20% FBS, 10% DMSO |
| Freezing Instructions | Cells are recommended to generate additional frozen stocks at early passages. Frozen stocks should be preserved in a designated cryopreservation medium or in 70% RPMI 1640 + 20% FBS + 10% DMSO (without antibiotic selection marker). 1. Prepare the freezing medium (70% RPMI 1640 + 20% FBS + 10% DMSO, without antibiotic selection marker) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250 x g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3 x10^6 cells/ml in chilled freezing medium. 6. Aliquot 1 ml of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage. |
| Gene Name | EGFR epidermal growth factor receptor [ Homo sapiens ] |
| Gene Symbol | EGFR |
| Synonyms | ERBB; HER1; mENA; ERBB1; PIG61 |
| Gene Description | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
| Gene ID | 1956 |
| UniProt ID | P00533 |
| mRNA Refseq | NM_005228.3 |
| Protein Refseq | NP_005219.2 |
| Chromosome Location | 7p12 |
| Function | ATP binding; MAP kinase kinase kinase activity; actin filament binding; double-stranded DNA binding; enzyme binding; epidermal growth factor-activated receptor activity; epidermal growth factor-activated receptor activity; identical protein binding; contributes_to nitric-oxide synthase regulator activity; protein binding; protein heterodimerization activity; protein phosphatase binding; protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; transmembrane signaling receptor activity; |
| Pathway | Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Arf6 signaling events, organism-specific biosystem; Axon guidance, organism-specific biosystem; |
| MIM | 131550 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
EGFR gene mutations like T790M and L858R cause osimertinib resistance in non-small cell lung cancer (NSCLC). These alterations complicate therapy, making resistance-overcoming techniques essential. The researchers explored the effectiveness of brigatinib against osimertinib-resistant EGFR mutants, including EGFR-T790M and C797S, through crystal structure analysis and mutagenesis assays. They identified novel mutations, such as L718M and G796S, which are resistant to all clinically available EGFR-TKIs. Additionally, the study demonstrated that BI-4020, a fourth-generation EGFR inhibitor, can overcome these resistance mutations but still faces challenges with minor mutations like L747P.
Figure 1. The researchers used ENU mutagenesis screening to identify resistance mutations in Ba/F3 cells expressing EGFR mutations, such as L718 and G796N, after treatment with brigatinib. (Suzuki M, et al., 2024)
Creative Biogene's Human EGFR-Del19/T790M/L858R Stable Cell Line-Ba/F3 is useful for NSCLC EGFR mutation-driven resistance studies. This cell line, intended to express prevalent NSCLC EGFR mutations, may be used to test new EGFR inhibitors and combination therapy against drug-resistant EGFR mutations.
A: Our human EGFR-Del19/T790M/L858R stable cell line has undergone rigorous purification and verification procedures to ensure its purity and stability. We use a variety of techniques to identify cell lines, including PCR, immunofluorescence staining, and Western blot. We also regularly perform cytological testing of cell lines to ensure they are free of hybridization or mutation.
A: Our human EGFR-Del19/T790M/L858R stable cell line has stable expression levels and has been widely used in anti-cancer drug resistance research. We provide detailed experimental data support that customers can refer to to evaluate the performance of our products.
A: Our human EGFR-Del19/T790M/L858R stable cell line has been widely used in anti-cancer drug screening experiments. We provide relevant literature support demonstrating the potential application of our products in anticancer drug screening.
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I recently used the Human EGFR-T790M Stable Cell Line-Ba/F3 for experiments, and the overall experience was excellent. This cell line stably expresses the T790M mutation of the human epidermal growth factor receptor (EGFR) and provides an important tool for studying EGFR-TKI resistance.
Human EGFR-T790M Stable Cell Line-Ba/F3 is a very efficient research model, especially suitable for studying the mechanism of EGFR-TKI resistance. This cell line stably expresses the T790M mutation of EGFR, simulating a common clinical resistance mechanism. Using this cell line for experiments, I obtained stable and reliable results. The service provided by the merchant was also very timely and professional, and they gave me full support and guidance.
The stability and consistency of the cell lines makes my research run more smoothly. The service quality provided by the boss is also very high. They responded quickly and provided professional technical support, which greatly improved my experimental efficiency.
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