Human TERT Lentivirus (EF1α, Puro)

Name: Human TERT Lentivirus (EF1α, Puro)
SKU: LVIM010Z

Cat.No. : LVIM010Z

Titer: ≥1*10^7 TU/mL / ≥1*10^8 TU/mL / ≥1*10^9 TU/mL Size: 100 ul/500 ul/1 mL

Storage: -80℃ Shipping: Frozen on dry ice

Inquire for Price

Lentivirus Particle Information

Quality Control

Cat. No.	LVIM010Z
Description	Lentivirus expressing untagged Human TERT under the control of EF1α promoter, and puromycin resistance marker for mammalian cell selection.
Target Gene	TERT
Species	Human
Application	Human TERT lentivirus (EF1α, Puro) has a variety of important applications in research and biotechnology, primarily related to the study and manipulation of cellular aging, immortalization, and gene expression. Here are some key applications: Cell Immortality: Introducing TERT (telomerase reverse transcriptase) into cells can maintain telomere length, effectively "immortalizing" cells by allowing them to divide indefinitely. This is particularly useful for creating stable cell lines for long-term experiments. Cancer Research: Many cancers exhibit upregulated telomerase activity, causing cancer cells to proliferate uncontrollably. Studying cells that overexpress TERT can provide insight into the mechanisms of tumorigenesis and cancer progression. It can also be used to screen for potential anticancer drugs that target telomerase activity. Stem Cell Biology: Stem cells have higher telomerase activity compared to differentiated cells. Using lentivirus expressing TERT, researchers can explore the role of telomerase in maintaining stem cell properties and study how telomere maintenance affects stem cell differentiation and regeneration. Gene Therapy Development: This system provides a model for understanding how telomerase can potentially be used for gene therapy applications, such as for the treatment of diseases associated with premature telomere shortening or degenerative diseases. Drug Discovery and Development: Stable cell lines created with human TERT lentivirus can be used for high-throughput screening of drugs that inhibit or activate telomerase. These screens could aid in the discovery of new therapeutic agents for diseases such as cancer and telomere syndromes.
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Quick Inquiry

Background

Publications

Q & A

Customer Reviews

Human telomerase reverse transcriptase (hTERT) is the catalytic subunit of telomerase and plays a critical role in maintaining telomere length as well as chromosome integrity and stability. The Human TERT Lentivirus (EF1α, Puro) is a sophisticated tool designed to facilitate the study of telomerase activity and the role of hTERT in a variety of cellular contexts. This lentiviral vector system allows for the stable introduction and expression of untagged human TERT under the control of the elongation factor-1 alpha (EF1α) promoter in mammalian cells. In addition, it includes a puromycin resistance marker for efficient selection of transduced cells.

The EF1α promoter is known for its ability to drive robust and ubiquitous expression in a variety of cell types, both dividing and non-dividing. This makes it an ideal choice for research applications that require consistent and reliable expression of hTERT. By utilizing an untagged version of human TERT, this lentivirus minimizes potential issues associated with tag-related alterations in protein function, resulting in a more accurate representation of native hTERT activity. One of the standout features of this lentiviral construct is the inclusion of a puromycin resistance gene. This feature allows direct selection of successfully transduced cells by puromycin treatment, ensuring that only cells expressing the human TERT gene survive.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

First Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *