EF1a-hTERT(Neo) Lentiviral Particles

EF1a-hTERT(Neo) Lentiviral Particles are a sophisticated genetic tool designed for the robust and stable expression of the human telomerase reverse transcriptase (hTERT) gene in a wide variety of mammalian cells. These replication-incompetent particles utilize the human Elongation Factor 1-alpha (EF1a) promoter, which is specifically chosen for its ability to drive high-level, constitutive transgene expression while remaining resistant to the transcriptional silencing that often affects CMV promoters in stem cells and primary cultures. The vector backbone includes a Neomycin (Neo) resistance marker, which facilitates the efficient selection of stable cell populations using G418 antibiotic treatment. Produced through a high-titer manufacturing process, these lentiviral particles ensure high transduction efficiency in both dividing and non-dividing cells, integrating the hTERT expression cassette directly into the host genome for permanent genetic modification.

The primary application of these lentiviral particles is the immortalization of primary cells, allowing researchers to bypass replicative senescence and create continuous cell lines that maintain the phenotypic characteristics of their original tissue. Unlike traditional immortalization methods involving viral oncogenes, hTERT expression preserves a more natural cellular state, making these models ideal for studies in aging, oncology, and regenerative medicine. Additionally, EF1a-hTERT(Neo) particles are widely employed in the development of cell-based assays for drug discovery and toxicology, where a consistent and renewable cell source is mandatory. They also serve as a vital tool in telomere biology research, enabling the exploration of telomerase activity in cell survival, DNA repair mechanisms, and long-term proliferative capacity. By providing a reliable platform for stable hTERT overexpression, these particles support the establishment of complex, longitudinal biological models that are essential for modern biomedical research.