EF1a-hTERT(RFP, Bla) Lentiviral Particles

EF1a-hTERT (RFP, Bla) Lentiviral Particles represent a highly efficient system for the stable expression of human Telomerase Reverse Transcriptase (hTERT) in mammalian cells. This specific construct is regulated by the elongation factor 1-alpha (EF1a) promoter, a constitutive driver known for maintaining high levels of transgene expression even in sensitive cell types where other promoters, such as CMV, may undergo epigenetic silencing. To facilitate ease of use, the particles integrate a Red Fluorescent Protein (RFP) as a visual reporter for monitoring transduction efficiency and a Blasticidin resistance marker for the rapid selection of stable integrants. These third-generation, VSV-G pseudotyped lentiviral particles are engineered for high biosafety and broad tropism, enabling the delivery of the hTERT gene into a diverse array of target cells, including primary cells, stem cells, and hard-to-transfect lines, while ensuring long-term genomic stability.

The application of these lentiviral particles is primarily focused on cell immortalization, providing researchers with a reliable method to extend the proliferative capacity of primary human cells. By counteracting the natural shortening of telomeres during cell division, hTERT expression allows cells to bypass replicative senescence, making it possible to establish stable, long-lived cell models that retain their original phenotypic traits. This is particularly valuable in fields such as cancer research, where telomerase activation is a key area of study, and in regenerative medicine, where expanded populations of primary cells are needed for therapeutic development. Additionally, the dual-marker system allows for precise cell sorting and the generation of homogeneous cell lines for high-throughput drug screening and functional genomic assays, significantly streamlining the workflow for longitudinal biological studies and complex tissue engineering projects.