CMV-hTERT(GFP, Bla) Lentiviral Particles

CMV-hTERT(GFP, Bla) lentiviral particles are sophisticated, multifunctional tools for cell reprogramming and longevity research. This advanced lentiviral vector system integrates three key components: the human telomerase reverse transcriptase (hTERT) gene driven by the EF1a promoter, the green fluorescent protein (GFP) reporter gene, and the blast fungicide resistance gene. The EF1a promoter ensures stable expression of hTERT in various mammalian cell types, including primary and difficult-to-transduce cells, and maintains long-term expression through genomic integration. The addition of GFP allows for immediate visual monitoring of successful transduction via fluorescence microscopy, enabling real-time monitoring of infection efficiency and spatial distribution without additional staining or fixation steps. Simultaneously, the blast fungicide resistance gene provides a highly efficient screening mechanism, enabling researchers to rapidly remove untransduced cells and establish pure clonal cell populations with extremely low background noise.

The primary application of these lentiviral particles is cell immortalization. By delivering the catalytic subunit of telomerase, hTERT, this system effectively extends telomeres and bypasses senescence, transforming transient cell cultures into stable proliferating cell lines while preserving key physiological functions. This capability is crucial for constructing disease models using patient-derived cells, such as fibroblasts or epithelial cells, which retain their original genetic background and can be used to study aging-related diseases or cancer progression. Beyond immortalization, the GFP reporter gene enables non-invasive tracking of transduced cells in co-culture systems, migration assays, or post-transplant in vivo studies, providing insights into cell behavior and interactions. Furthermore, this tool facilitates research in telomere dynamics, DNA damage responses, and regenerative medicine, where immortalized cells can serve as a stable platform for tissue engineering scaffold development.