EF1a-hTERT(RFP, Puro) Lentiviral Particles

The EF1a-hTERT (RFP, Puro) Lentiviral Particles represent a sophisticated tool for the stable expression of human telomerase reverse transcriptase in mammalian systems. Utilizing the elongation factor-1 alpha (EF1a) promoter, these particles provide consistent and high-level transcription, specifically in cell types where other promoters, such as CMV, may be prone to epigenetic silencing. The vector is engineered with a dual-reporter and selection system, featuring Red Fluorescent Protein (RFP) for non-invasive visualization and a Puromycin resistance gene to facilitate the generation of pure, stable cell lines. These lentiviral particles are provided in a ready-to-use, high-titer format and are typically pseudotyped with the VSV-G protein, allowing for a broad tropism that covers almost all mammalian cell varieties, including non-dividing and primary cells.

These lentiviral particles are predominantly employed in cell engineering to achieve cellular immortalization. By introducing hTERT, researchers can extend the lifespan of primary cells indefinitely while preserving their original physiological properties and karyotypic stability. This makes them indispensable for creating robust disease models, particularly in studies involving human fibroblasts, epithelial cells, or mesenchymal stem cells. Beyond immortalization, the integrated RFP marker allows for the precise monitoring of cell population dynamics and migration in complex microenvironments or co-culture systems. The system is also highly effective for creating long-term cultures required for pharmaceutical toxicology assays and vaccine development, where consistent cellular performance over multiple passages is critical. By providing a reliable method for stable gene integration, these particles streamline the workflow from initial transduction to high-throughput functional analysis.