CMV-hTERT(RFP, Puro) Lentiviral Particles

CMV-hTERT(RFP, Puro) lentiviral particles represent a cutting-edge tool for gene manipulation in cell research. This genetically engineered lentiviral vector system expresses the human telomerase reverse transcriptase (hTERT) gene under the control of a highly efficient EF1α promoter, ensuring stable and sustained expression across multiple cell types. The introduction of the RFP (red fluorescent protein) reporter gene provides a simple visual marker, enabling real-time tracking of transduction efficiency and monitoring of transgene expression without additional staining or complex assays. Furthermore, the puromycin resistance gene provides a robust screening mechanism, allowing researchers to obtain stably transduced purified cell populations with minimal effort. The lentiviral backbone further enhances the system's versatility, enabling efficient transduction of both dividing and non-dividing cells, including difficult-to-transfect primary cells.

The primary application of CMV-hTERT(RFP, Puro) lentiviral particles lies in cell immortalization, a key technology for overcoming the natural replication and senescence of primary cells. By introducing the catalytic subunit hTERT of telomerase, this system maintains telomere length, thereby extending cell lifespan and effectively bypassing the Hayflick limit. This is particularly important for establishing stable cell lines from patient-derived samples, tumor tissues, or rare cell populations, which have limited proliferative capacity in vitro. Researchers have also used the system to simulate age-related diseases, cancer progression (characterized by telomerase reactivation), and regenerative medicine applications. The vector's dual selectivity ensures experimental reproducibility and reduces variability, making it an indispensable tool in high-throughput screening, long-term biomarker studies, and therapeutic development processes that demand high consistency in cellular behavior.