Cat.No. : AD00397Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00397Z |
| Description | Human Adenovirus Type5 (dE1/E3) expressing telomerase reverse transcriptase under CMV promoter. No fusion tag, pre-made adenovirus, ready to ship and ready to use format. |
| Target Gene | TERT |
| Product Type | Adenoviral particle |
| Insert | TERT |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
| Gene Name | Telomerase reverse transcriptase |
| Gene Symbol | TERT |
| Gene ID | 7015 |
| mRNA Refseq | BC172541 |
Telomerase reverse transcriptase (TERT) has been reported to protect cells from ischemic injury in many diseases. However, the protective role of TERT in myocardial ischemia-reperfusion injury (MIRI) is still unclear. Here, rat myocardial H9c2 cells were subjected to oxygen and glucose deprivation (OGD) followed by reperfusion to simulate MIRI in vivo. TERT expression increased in H9c2 cells after OGD. A TERT overexpression model was constructed by adenovirus infection. After OGD, TERT overexpression promoted H9c2 cell proliferation, attenuated cell apoptosis, and enhanced autophagy. These studies suggest that TERT plays a protective role by promoting cardiomyocyte proliferation, inhibiting cell apoptosis, and enhancing autophagy after MIRI. Further studies on TERT are expected to reveal new therapeutic targets for myocardial ischemia-reperfusion injury.
Cultured myocardial cells (H9c2) were divided into 4 groups: normoxia control group (CON), cells treated with oxygen and glucose deprivation (OGD), cells infected with empty vector adenovirus and treated with OGD (OGD + EMPTY), cells infected with TERT overexpression adenovirus and treated by OGD (OGD + TERT). Western blotting was used to detect the expression of TERT in cardiomyocytes of each group 48 h after OGD. The expression levels of TERT in OGD and OGD + EMPTY groups were significantly higher than those in CON group (Figure 1), indicating that ischemia-reperfusion injury induced the expression of TERT in cardiomyocytes. Moreover, the expression of TERT in the OGD + TERT group was further significantly higher than that in the OGD and OGD + EMPTY groups (Figure 1). There was no significant difference in the expression levels of TERT between the OGD group and the OGD + EMPTY group (Figure 1). This demonstrated that the expression of TERT in myocardial cells was enhanced by infection with TERT overexpression adenovirus after OGD.
Figure 1. Expression of TERT in H9c2 cells. (Fang Z, et al., 2020.)
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The TERT adenovirus effectively extended replicative lifespan in our primary cells. The virus was high-titer and ready-to-use, saving us weeks of optimization.
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