Luciferase

Official Full Name

Luciferase

Background

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Synonyms

Luciferase; ec 1.13.12.7; Firefly; Luciferin 4 monooxygenase;

Transfected Stable Cell Lines
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Detailed Information

The phenomenon of bioluminescence has been observed in many different organisms including bacteria, fungi, algae, fish, squid, shrimp and insects. Luminous organisms produce light by an enzymatic reaction of a luciferase (=enzyme) with a luciferin (=substrate). Light-emitting reactions are quite distinct among luminous organisms, but in each case, the reaction is an oxidation process with molecular oxygen and is a conversion of chemical energy into light. The various luciferase genes have been isolated and adapted as reporter genes. Firefly luciferase (FLuc) has been studied for the last 50 years and proven to be a useful enzyme. It has been widely used in molecular and cell biology, particularly for the quantification of ATP and as a reporter enzyme of gene expression.

One of the main reasons for the widespread use of luciferase in bioassays is that, unlike fluorescence, luminescence does not require excitation light energy, so lowering the background signal to provide a sensitive assay with a high signal-to-background ratio. Elimination of an excitation light source also prevents interference by compound fluorescence and fluorophore photobleaching. Thus, luminescence assays can be very sensitive, despite significantly weaker signal intensity than fluorescence. For cell-based assays, the use of FLuc or Renilla luciferase (RLuc) enzymes as reporters enable the measurement of dynamic changes in reporter transcription levels because the intracellular protein half-lives of these luciferase enzymes are relatively short compared to nonenzymatic fluorescent protein reporters (e.g., GFP).

Figure 1. Principle of a simple luciferase reporter assay.

Gene function analysis, target discovery and validation, compound screening, and assay development often need cell-based assays. Stable cell lines that are engineered to express a gene of interest by transgene integrations into the host genome provide an efficient approach to conduct such analysis. Creative Biogene can help researchers save time and labor in generating and validating stable cell lines so scientists can spend more effort on solving the big questions. We offer a wide-selection of validated luciferase reporter cell lines that measure transcription factor activity as a read-out for various signaling pathways.

Features:

Consistent - TF reporter construct is stably integrated into the genome to avoid experimental/cell-to-cell variations.
High sensitive and responsive - Each cell line is validated to induce strong reporter signal in response to stimuli.
Routine mycoplasma testing - All cell lines tested negative for mycoplasma.
Time saving - Cell line can be used for experiments right away to study different signaling pathways.

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