RFP Reporter Cell Line - Hep3B

Hep3B is a well-established human hepatocellular carcinoma (HCC) cell line derived from a liver tumor of an 8-year-old male patient. This cell line exhibits epithelial cell morphology and adherent growth characteristics. A notable feature of Hep3B cells is their ability to secrete alpha-fetoprotein (AFP) and albumin, two key biomarkers for liver function and cancer progression. Furthermore, these cells retain the hepatitis B virus (HBV) genome, making them a valuable model for studying HBV-related hepatocarcinogenesis. Due to its relevance to human liver cancer biology, the Hep3B cell line is widely used in oncology, virology, and drug discovery research.

The RFP reporter gene cell line – Hep3B – has been genetically engineered to express red fluorescent protein (RFP), enabling real-time visualization and tracking of cell dynamics in live-cell imaging, migration assays, and in vivo models. This modification allows researchers to monitor tumor growth, metastasis, and response to treatment with high precision. In drug screening, the RFP reporter system can rapidly assess compound efficacy by quantifying changes in fluorescence intensity, which correlate with cell viability or apoptosis. Additionally, this cell line can be used in co-culture experiments with other labeled cell types to study tumor microenvironment interactions, such as angiogenesis and immune evasion. The RFP Hep3B cell line also supports long-term studies in animal models, where non-invasive imaging techniques can track metastatic spread and treatment effects.