Cat.No. : CSC-RR0318 Host Cell: MC3T3
| Cat. No. | CSC-RR0318 |
| Description | MC3T3-RFP cell line is clonally derived from MC3T3 cell line which was transduced by a lentiviral vector containing RFP and puromycin resistance gene. RFP is expressed as free cytoplasmatic proteins in these MC3T3-RFP stable cells. It is a ready-to-use in vitro model for cell-based assay applications. |
| Background | The RFP Reporter Cell Line-MC3T3 |
| Gene | RFP |
| Host Cell | MC3T3 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Source | MC3T3 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Autophagy, a dynamic and highly conserved cellular process, is notably upregulated in cells under diverse stress conditions. Researchers investigated the role of death-associated protein 1 (DAP1) in modulating autophagy in Graves' disease (GD)-induced osteoporosis (OP) using the RFP Reporter Cell Line-MC3T3. Bone proteomics analysis identified DAP1 as a key protein. Genetic manipulation of DAP1 in MC3T3-E1 cells revealed its effects on autophagy and osteogenic markers. Electron microscopy and MAP1LC3/LC3 fluorescence were used to visualize autophagic vacuoles. Results showed that DAP1 overexpression inhibited autophagy and osteogenesis markers, while knockdown reversed these effects. In vivo, DAP1 knockdown increased bone mass in GD mice. DAP1 was found to regulate autophagy and osteogenesis via the ATG16L1–LC3 axis in osteoblasts, highlighting its role in GD-induced osteoporosis.
Figure 1. The impact of DAP1 on autophagic vacuoles and flux in MC3T3-E1 cells was investigated. RFP Reporter Cell Line-MC3T3 was utilized for fluorescence imaging of autophagic vacuoles. Alterations in autophagic vacuole numbers and distribution were observed, providing insights into DAP1's role in autophagy regulation. These findings contribute to understanding cellular mechanisms underlying bone metabolism. (Gao M, et al., 2023)
A: RFP Reporter Cell Line-MC3T3 serves as a valuable tool for studying bone cell differentiation, proliferation, and morphology. The red fluorescent protein allows for the visualization and tracking of osteogenic lineage cells, providing insights into bone tissue formation and regeneration.
A: The RFP reporter in HEK293 cells serves as a valuable tool for investigating gene expression, protein dynamics, and cellular interactions in various biological contexts, facilitating research in areas such as cell biology, developmental biology, and drug discovery. Its versatility enables the development of novel assays and screening platforms for studying complex biological phenomena and identifying potential therapeutic targets.
A: RFP Reporter Cell Line-MC3T3 is suitable for high-throughput screening assays in bone biology research. The red fluorescence allows for efficient and automated detection of osteogenic cell activity, enabling rapid screening of compounds or treatments for their effects on bone cell behavior.
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The RFP Reporter Cell Line consistently delivers bright signals, ensuring clear visualization and accurate quantification of cellular events. From investigating osteogenesis to modeling bone diseases, this cell line serves as a valuable tool for advancing our understanding of skeletal biology and developing therapeutic strategies. Reliable performance!
This cell line facilitates detailed examination of osteoblast behavior, differentiation, and interactions in vitro and in vivo. Its stable RFP expression streamlines experimental workflows, providing a straightforward approach to studying bone development and pathology.
Vibrant visualization! The RFP Reporter Cell Line in MC3T3 cells brings fluorescence imaging to life, enabling vivid tracking and analysis of cellular processes in bone research.
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