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RFP Reporter Cell Line - HCoF-IM

RFP Reporter Cell Line - HCoF-IM

Cat.No. :  CSC-RR00617 Host Cell:  HCoF-IM

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Cell Culture Information

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Cat. No. CSC-RR00617
Description This cell line is an immortalized human colonic fibroblast cell line (HCoF) expressing RFP reporter gene. It is an useful cell model for studying the mechanisms of colon carcinogenesis.
Gene RFP
Host Cell HCoF-IM
Host Cell Species Homo sapiens (Human)
Stability Validated for at least 10 passages
Reporter Type Fluorescent protein
Application

1. Gene expression studies

2. Protein localization

3. Drug screening and toxicology

4. Live cell imaging

Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Shipping Dry ice
Storage Liquid nitrogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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